Extended Myositis Panel
Ordering Recommendation

May be useful for differential evaluation of polymyositis, dermatomyositis, necrotizing autoimmune myopathy, or overlap syndromes associated with connective tissue disease.

Qualitative Immunoprecipitation/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot
7-18 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum Separator Tube (SST). 
Specimen Preparation
Separate from cells ASAP or within 2 hours of collection. Transfer three 1 mL serum aliquots to ARUP Standard Transport Tubes. (Min: 0.5 mL/aliquot) 
Storage/Transport Temperature
Unacceptable Conditions
Hemolyzed, hyperlipemic, icteric, heat-treated or contaminated specimens. 
Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year 
Reference Interval
Test Number
Reference Interval
2012074SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
Test Number
Reference Interval
SSA-52 (Ro52) (ENA) Antibody, IgG29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

0050470Smith/RNP (ENA) Antibody, IgG
29 AU/mL or lessNegative
30-40 AU/mLEquivocal
41 AU/mL or greaterPositive

0099592Jo-1 Antibody, IgG
29 AU/mL or lessNegative
30-40 AU/mLEquivocal
41 AU/mL or greaterPositive

2012173Fibrillarin (U3 RNP) Antibody, IgGNegative
2003040PM/Scl-100 Antibody, IgG by ImmunoblotNegative
 Mi-2 (nuclear helicase protein) AntibodyNegative
 PL-7 (threonyl-tRNA synthetase) AntibodyNegative
 PL-12 (alanyl-tRNA synthetase) AntibodyNegative
 P155/140 AntibodyNegative
 EJ (glycyl-tRNA synthetase) AntibodyNegative
 Ku AntibodyNegative
 SRP (Signal Recognition Particle) AbNegative
 OJ (isoleucyl-tRNA synthetase) AntibodyNegative
 SAE1 (SUMO activating enzyme) AbNegative
 MDA5 (CADM-140) AbNegative
 NXP2 (Nuclear matrix protein-2) AbNegative
 TIF-1 gamma (155 kDa) AbNegative

Interpretive Data
Refer to report.

Compliance Statement D: For laboratory tests using a manufactured RUO kit. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Antibodies: Mi-2, PL-7, PL12, P155/140, EJ, Ku, OJ, PM/Scl, SRP, Smith/RNP, Ro52, Ro60, Jo-1, U3 Fib, SAE1, NXP2, MDA5, TIF1-gamma
Hotline History
View Hotline History
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0050470Smith/RNP (ENA) Ab, IgG33983-8
0050691SSA-52 (Ro52) (ENA) Antibody, IgG31625-7
0099592Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG33571-1
2003041PM/Scl 100 Antibody, IgG81732-0
2010852Mi-2 (nuclear helicase protein) Antibody18485-3
2010853PL-7 (threonyl-tRNA synthetase) Antibody33772-5
2010854PL-12 (alanyl-tRNA synthetase) Antibody33771-7
2010855P155/140 Antibody
2010856EJ (glycyl-tRNA synthetase) Antibody45149-2
2010857Ku Antibody18484-6
2010859SRP (Signal Recognition Particle) Ab33921-8
2010860OJ (isoleucyl-tRNA synthetase) Antibody45152-6
2012055SSA-60 (Ro60) (ENA) Antibody, IgG53019-6
2012174Fibrillarin (U3 RNP) Ab, IgG49963-2
2013994Myositis Panel Interpretive Data48767-8
3001786SAE1 (SUMO activating enzyme) Ab82440-9
3001787MDA5 (CADM-140) Ab82424-3
3001788NXP2 (Nuclear matrix protein-2) Ab82426-8
3001789TIF-1 gamma (155 kDa) Ab82448-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.