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Hepatitis C Virus (HCV) by Quantitative NAAT
3000572
Ordering Recommendation

Preferred single test to confirm hepatitis C virus (HCV) infection following positive HCV antibody screen.

Mnemonic
HEPC QNT
Methodology
Quantitative Transcription Mediated Amplification
Performed
Sun-Sat
Reported
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), Pink (K2EDTA), Yellow (ACD), Plasma Preparation Tube (PPT), or Serum Separator Tube (SST). 
Specimen Preparation
Separate from cells within 6 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1.2 mL) 
Storage/Transport Temperature
Frozen. 
Unacceptable Conditions
Heparinized specimens. 
Remarks
 
Stability
After separation from cells: Ambient: Unacceptable; Refrigerated: 5 days; Frozen: 2 months 
Reference Interval
Not Detected
Interpretive Data
Normal range for this assay is "Not Detected".
The quantitative range of this assay is 10 - 100,000,000 IU/mL (1.0 - 8.0 log IU/mL).

Lower limit of quantitation (LLoQ):
10 IU/mL (1.0 log IU/mL)
LLoQ values do not apply to diluted specimens.
A result of "Not Detected" does not rule out the presence of inhibitors in the patient specimen or hepatitis C virus RNA concentrations below the level of detection of the test. Care should be taken when interpreting any single viral load determination.

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).

Note
The limit of quantification for this RNA assay is 10 IU/mL (1.0 log IU/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "Not Detected" If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "< 10 Detected".

Specimens received with less than minimum volume for testing will automatically be run with a dilution according to the guidelines below:
-Specimens with 240-700 µL will be diluted 1:3 resulting in a quantitative range of 30 - 300,000,000 IU/mL (1.48-8.48 log IU/mL) .

This test is intended for use as an aid in the management of HCV-infected patients undergoing anti-viral therapy in conjunction with clinical and laboratory markers of infection. This test is also used in assessing HCV RNA levels at baseline, during treatment, at the end of treatment, and at the end of follow up of treatment to determine sustained or non-sustained viral response.
Hotline History
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Components
Component Test Code*Component Chart NameLOINC
3000573HCV Qnt by NAAT (IU/mL)11011-4
3000574HCV Qnt by NAAT (log IU/mL)38180-6
3000966HCV Qnt by NAAT Interp11259-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HCV monitoring
  • HCV quantitative
  • HCV Quantitative PCR
  • HCV Quantitative TMA
  • HCV Screen confirmation
  • HCV Viral Load
  • Nucleic Acid Amplification Test (NAAT)
  • Viral load monitoring