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Maternal Serum Screen, Alpha Fetoprotein, hCG, Estriol, and Inhibin A (Quad)
3000143
Ordering Recommendation

Second-trimester screening test for trisomy 21 (Down syndrome), trisomy 18, and open neural tube defects.

Mnemonic
MS QUAD
Methodology
Quantitative Chemiluminescent Immunoassay
Performed
Sun-Sat
Reported
2-3 days
New York DOH Approval Status
This test is New York DOH approved.
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Specimen must be drawn between 14 weeks, 0 days and 24 weeks, 6 days gestation. The recommended time for maternal serum screening is 16 to 18 weeks gestation. 
Collect
Serum Separator Tube (SST) or Plain Red. 
Specimen Preparation
Separate from cells ASAP or within 2 hours of collection. Transfer 3 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Plasma. Hemolyzed specimens. 
Remarks
Submit with Order: Patient's date of birth, current weight, due date, dating method (US, LMP), number of fetuses present, patient's race, if the patient was diabetic at the time of conception, if there is a known family history of neural tube defects, if the patient has had a previous pregnancy with a trisomy, if the patient is currently smoking, if the patient is taking valproic acid or carbamazepine (Tegretol), if this is a repeat sample, and the age of the egg donor if in vitro fertilization. 
Stability
After separation from cells: Ambient: 72 hours; Refrigerated: 2 weeks; Frozen: 1 year (Avoid repeated freeze/thaw cycles.) 
Reference Interval
By report
Interpretive Data
Refer to report.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
This test is used to screen for fetal risk of Down syndrome (trisomy 21), trisomy 18, and Open Neural Tube Defect (ONTD, spina bifida).
Hotline History
View Hotline History
Components
Component Test Code*Component Chart NameLOINC
0080021Patient's AFP1834-1
0080241Estimated Due Date11778-8
0080267MoM for DIA35738-4
0080268Patient's DIA23883-2
0080918MoM for AFP20450-3
0080920Maternal Screen Interpretation49586-1
0080922Specimen19151-0
0080923Dating21299-3
0080924Insulin Req Maternal Diabetes44877-9
0080925Family Hx Neural Tube Defect8670-2
0080926Maternal Race21484-1
0080927Number of Fetuses11878-6
0080932Maternal Age At Delivery21612-7
0080941Patient's uE32250-9
0080943MoM for uE320466-9
0081158Family History of Aneuploidy32435-0
3000165Smoking64234-8
3000171EER Maternal Serum, Quad11526-1
3000261Patient's hCG, 2nd Trimester19080-1
3000262hCG MoM, 2nd Trimester20465-1
3000263Gestational Age Calculated at Collection18185-9
3000264Maternal Weight29463-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • AFP 4 Marker Screen
  • AFP MS4 (Quad)
  • Alpha Fetoprotein, hCG, Estriol, and Inhibin
  • Maternal Screening, AFP 4 Marker
  • QUAD
  • Quad AFP
  • Quad screening