Alpha/Beta Double-Negative T-Cells for Autoimmune Lymphoproliferative Syndrome
2014513
Ordering Recommendation
Support the diagnosis of autoimmune lymphoproliferative syndrome (ALPS).
Mnemonic
ALPS ABDNT
Methodology
Quantitative Flow Cytometry
Performed
Sun-Sat
Reported
1-3 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
Specimen Required
- Patient Preparation
- Collect
- Green (Sodium or Lithium Heparin), Lavender (EDTA), or Pink (K2EDTA)
- Specimen Preparation
- Transport 5 mL whole blood. (Min: 0.5 mL)
- Storage/Transport Temperature
- CRITICAL ROOM TEMPERATURE
- Unacceptable Conditions
- Clotted or hemolyzed specimens
- Remarks
- Specimens must be analyzed within 48 hours of collection
- Stability
- Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval
Reports include age appropriate reference intervals and interpretation.
Test Number | Components | Age: 2-18 years old | Age 18-69 years old |
Absolute alpha/beta TCR+ DNT | 0-46 (cells/uL) | 0-32 (cells/uL) | |
Absolute alpha/beta TCR+ DNT B220+ | 0-5 (cells/uL) | 0-6 (cells/uL) | |
% alpha/beta TCR+ DNT | 0-3 (%) | 0-2 (%) | |
% alpha/beta TCR+ DNT B220+ | 0-0.3 (%) | 0-0.4 (%) |
Interpretive Data
Reported percentages reflect percentage of CD3 positive lymphocytes. The hallmark for a diagnosis of Autoimmune Lymphoproliferative Syndrome (ALPS) is an increased concentration of CD3+ T-cells negative for CD4 and CD8 (double-negative T-cells [DNT]) and positive for the alpha/beta T-cell receptor (TCR). B220 expression on alpha/beta TCR+DNT cells is a sensitive and specific marker for ALPS and is associated with mutations in the FAS gene.
Abnormal results should be correlated with clinical history and confirmed by additional testing for defective in vitro lymphocyte apoptosis and for mutations in the FAS gene.
Abnormal results should be correlated with clinical history and confirmed by additional testing for defective in vitro lymphocyte apoptosis and for mutations in the FAS gene.
Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
Note
Hotline History
View Hotline History
CPT Code(s)
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
2014514 | Absolute alpha/beta TCR+ DNT | |
2014515 | Absolute alpha/beta TCR+ DNT B220+ | |
2014516 | % alpha/beta TCR+ DNT | |
2014517 | % alpha/beta TCR+ DNT B220+ |
Aliases