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Antimicrobial Susceptibility - Carbapenemase Gene Detection by PCR
2014277
Ordering Recommendation

Detect the presence of predominant carbapenemase genes (blaKPC, blaNDM, blaVIM, blaOXA-48, blaIMP) responsible for resistance to carbapenem and other beta-lactam antibiotics.

Mnemonic
CARBAR PCR
Methodology
Qualitative Polymerase Chain Reaction
Performed
Sun-Sat
Reported
1-4 days
N/A
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Actively growing Enterobacteriaceae, Pseudomonas aeruginosa, or Acinetobacter baumannii in pure culture. 
Specimen Preparation
Transport sealed container with pure culture on agar slant/bacterial transport media. Place each specimen in an individually sealed bag. 
Storage/Transport Temperature
Room temperature. 
Unacceptable Conditions
Mixed cultures or non-viable organisms. 
Remarks
Isolate identification (for cultures) and specimen source required. 
Stability
Ambient: 1 week; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
Not Detected
Interpretive Data
This assay detects five carbapenemase gene families (blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP) encoding enzymes that may confer resistance to carbapenem and other beta-lactam antibiotics. This assay is intended for use as an aid to infection control in the detection of carbapenem-resistant bacteria and is not intended to guide or monitor treatment of infection. A negative result does not exclude the presence of other resistance mechanisms or assay-specific nucleic acid in concentrations below the level of detection.

Note
An additional processing fee will be billed for all isolates not submitted in pure culture, as indicated in the specimen requirements.

If species identification is not provided, identification will be performed at ARUP. Additional charges apply.

This assay will generate a negative IMP result when testing samples containing IMP-7, IMP-13 or IMP-14 gene sequences, and may detect IMP-4 at reduced sensitivity.
Components
Component Test Code*Component Chart NameLOINC
2014278KPC gene by PCR49617-4
2014279NDM gene by PCR73982-1
2014280OXA-48 gene by PCR63368-5
2014281VIM gene by PCR63368-5
2014282IMP gene by PCR63368-5
2014283CarbaR Interpretation48767-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • carbapenem-resistance
  • carbapenemase
  • CRE
  • Enterobacteriaceae
  • IMP
  • Klebsiella pneumoniae carbapenemase
  • KPC
  • KPCPCR
  • MA KPCPCR
  • MDR
  • multi-drug resistance
  • NDM
  • NewDelhi metallo-B-lactamase
  • OXA-48
  • resistance mechanism
  • VIM