Polymyositis and Dermatomyositis Panel
Ordering Recommendation

May be useful for the evaluation of patients with progressive proximal muscle weakness and/or cutaneous manifestations suggestive of dermatomyositis and/or associated connective tissue disease.

Qualitative Immunoprecipitation/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot
Mon, Tue, Thu, Fri
7-15 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum Separator Tube (SST). 
Specimen Preparation
Separate from cells ASAP or within 2 hours of collection. Transfer two 1 mL serum aliquots to ARUP Standard Transport Tubes. (Min: 0.5 mL/aliquot) 
Storage/Transport Temperature
Unacceptable Conditions
Hemolyzed, hyperlipemic, icteric, heat-treated or contaminated specimens. 
Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year 
Reference Interval
Test Number
Reference Interval
0099592Jo-1 Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
 PL-7 (threonyl-tRNA synthetase) AntibodyNegative
 PL-12 (alanyl-tRNA synthetase) AntibodyNegative
 EJ (glycyl-tRNA synthetase) AntibodyNegative
 SRP (Signal Recognition Particle) AbNegative
 OJ (isoleucyl-tRNA synthetase) AntibodyNegative
 Mi-2 (nuclear helicase protein) AntibodyNegative
 P155/140 (TIF1-gamma) AntibodyNegative
 SAE1 (SUMO activating enzyme) AntibodyNegative
 MDA5 (CADM-140) AntibodyNegative
 NXP-2 (Nuclear matrix protein-2) AbNegative
 TIF1-gamma AntibodyNegative

Interpretive Data
Refer to report.

Compliance Statement D: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Antibodies:  PL-7, PL12, EJ, OJ, SRP, Jo-1, Mi-2, P155/140, SAE1, MDA5, NXP2, TIF1-gamma
CPT Code(s)
83516 x11; 86235
Component Test Code*Component Chart NameLOINC
0099592Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG14235-6
2010852Mi-2 (nuclear helicase protein) Antibody18485-3
2010853PL-7 (threonyl-tRNA synthetase) Antibody33772-5
2010854PL-12 (alanyl-tRNA synthetase) Antibody33771-7
2010855P155/140 (TIF1-gamma) Antibody
2010856EJ (glycyl-tRNA synthetase) Antibody45149-2
2010859SRP (Signal Recognition Particle) Ab33921-8
2010860OJ (isoleucyl-tRNA synthetase) Antibody45152-6
2013962SAE1 (SUMO activating enzyme) Antibody
2013963NXP-2 (Nuclear matrix protein-2) Ab
2013964MDA5 (CADM-140) Antibody
2013965TIF1-gamma Antibody
2013997Myositis Interpretive Information48767-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.