Use for patients whose symptoms began, or whose documented exposure occurred, at least 14 days prior to testing. Should also be used as followup for patients with negative serum and urine results from molecular testing performed less than 14 days after symptom onset.
- Patient Preparation
- Serum Separator Tube (SST).
- Specimen Preparation
- Separate from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1.0 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute or convalescent."
- Storage/Transport Temperature
- Unacceptable Conditions
- Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.
- After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
The possibility of false-positive or false-negative results must be considered. RT-PCR testing on both a serum and urine specimen is recommended by the Centers for Disease Control and Prevention (CDC) to rule out false-negative IgM results in patients experiencing symptoms for less than 2 weeks. Specimens collected for IgM testing greater than or equal to 2 weeks after symptom onset do not require any additional testing. For more information, please review the current clinical guidelines for Zika virus testing at: www.cdc.gov/zika/.
|Component Test Code*||Component Chart Name||LOINC|
|2014017||Zika Virus IgM Ab Capture (MAC), ELISA||80824-6|
|2014019||Relevant Zika virus Exposure?|
|2014020||Symptoms consistent with Zika virus?||75325-1|