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Zika Virus IgM Antibody Capture (MAC), by ELISA
2013942
Ordering Recommendation

Use for patients whose symptoms began, or whose documented exposure occurred, at least 14 days prior to testing. Should also be used as followup for patients with negative serum and urine results from molecular testing performed less than 14 days after symptom onset.

Mnemonic
ZIKA M
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Mon, Wed, Fri
Reported
1-6 days
New York DOH Approval Status
This test is New York DOH approved.
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum Separator Tube (SST). 
Specimen Preparation
Separate from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1.0 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute or convalescent." 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Negative
Interpretive Data
The ZIKV Detect IgM Capture ELISA assay is intended for in vitrodiagnostic use under FDA Emergency Use Authorization (EUA). This test has not been FDA cleared or approved. In compliance with this authorization, please visit https://aruplab.com/zika for more information and to access the applicable information sheets.

The possibility of false-positive or false-negative results must be considered. RT-PCR testing on both a serum and urine specimen is recommended by the Centers for Disease Control and Prevention (CDC) to rule out false-negative IgM results in patients experiencing symptoms for less than 2 weeks. Specimens collected for IgM testing greater than or equal to 2 weeks after symptom onset do not require any additional testing. For more information, please review the current clinical guidelines for Zika virus testing at: www.cdc.gov/zika/.

Components
Component Test Code*Component Chart NameLOINC
2014017Zika Virus IgM Ab Capture (MAC), ELISA80824-6
2014018Pregnant?66174-4
2014019Relevant Zika virus Exposure?
2014020Symptoms consistent with Zika virus?75325-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases