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Candida FKS Drug Resistance by Sequencing
2013901
Ordering Recommendation

Detect known echinocandin resistance mutations in C. albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis. If Candida species is not available, order Candida Species by PCR with Reflex to FKS Drug Resistance by Sequencing (2013784).

Mnemonic
FKS SEQ
Methodology
Polymerase Chain Reaction/Sequencing
Performed
Sun, Mon, Wed
Reported
3-5 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Body fluid, tissue, or pure isolate of Candida species on potato dextrose agar (PDA), sabouraud dextrose agar, sheep blood agar, chocolate agar, or inhibitory mold agar. 
Specimen Preparation
Body Fluid: Transfer 1 mL body fluid to a sterile container. (Min: 0.5 mL)
Tissue: Transfer to a sterile container and freeze immediately.
Isolate: Transport sealed container with pure isolate on solid media. Place each specimen in an individually sealed bag. 
Storage/Transport Temperature
Body Fluid or Tissue: Frozen.
Isolate: Refrigerated. 
Unacceptable Conditions
Plasma or serum. Mixed cultures or isolates other than suspected Candida species. Candida species identified as C. dubliniensis. Isolates with no visible colonies. Isolates plated on Chromagar chromogenic culture media. 
Remarks
Candida species identification is required. Specimen source is required. 
Stability
Body Fluid: Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks
Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 2 weeks
Isolate: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: Unacceptable 
Reference Interval
Interpretive Data
This assay detects known resistance mutations in C. albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis by sequencing. The FKS1 and FKS2 genes are sequenced. Mutations associated with resistance to Echinocandins are reported. Mutations in subpopulations below 20 percent of total may not be detected.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
If the Candida species is other than C. albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis, testing will be canceled. If Candida species is not available, order Candida Species by PCR with Reflex to FKS Drug Resistance by Sequencing (ARUP test code 2013784). This test may be unsuccessful if the specimen or isolate does not contain C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis, or if multiple Candida species are present.
Hotline History
View Hotline History
Components
Component Test Code*Component Chart NameLOINC
2013785Candida Species Source31208-2
2013902FKS Drug Resistance by Sequencing
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases