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Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response
2013117
Ordering Recommendation

Primarily for research and to support attempts to understand the pathogenesis of immune, infectious, allergic, or inflammatory disorders.

Mnemonic
LAM CYT
Methodology
Cell Culture/Multiplex Bead Assay
Performed
Tue-Fri
Reported
9-10 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient. 
Collect
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be collected within 48 hours of test. 
Specimen Preparation
Transport 20 mL whole blood (patient) AND 20 mL whole blood (control) in original collection tubes. (Min: 14 mL (patient) AND 14 mL (control)) Do not refrigerate or freeze.
LIVE CELLS REQUIRED.
Infant Minimum: 3 mL (patient) AND 14 mL (control). 
Storage/Transport Temperature
CRITICAL ROOM TEMPERATURE. 
Unacceptable Conditions
Yellow (ACD Solution B). Specimens in transport longer than 48 hours. 
Remarks
 
Stability
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data
Candida and tetanus antigens are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these antigens is determined by 3H-thymidine incorporation.

Phytohemagglutinin, concanavalin A and pokeweed mitogen are tested independently in lymphocyte culture. Lymphocyte proliferation in response to the non-specific mitogens phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweed (PW) are determined by 3H-thymidine incorporation.

Results are reported as counts per minute (CPM) mitogen stimulated versus a control culture and a stimulation Index (SI) which represents the ratio of CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.

SI*=Stimulation Index (CPM Mitogen/CPM Media alone)

Antigens (Candida albicans, tetanus toxoid) and mitogens (phytohemagglutinin, concanavalin A, pokeweed) are tested independently in lymphocyte culture. Peripheral Blood Mononuclear Cell (PBMC) cytokine production responses to these antigens and mitogens are determined by quantitative multiplex bead assay. Results are used to understand the pathophysiology of immune, infectious, or inflammatory disorders, or may be used for research purposes.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
The following cytokines (and receptor) are reported: IL-2, sIL-2R (sCD25), IL-4, IL-5, IL-10, IL-13, IL-1b, IL-6, IL-8, IL-17, TNF-a, and IFN-g. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s)
86353 x5; 83520 x12
Components
Component Test Code*Component Chart NameLOINC
0096056Lymphocyte Ag and Mitogen Panel59063-8
2013112Cytokine Production, Ags and Mitogens53810-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Blastogenesis Antigens
  • Blastogenesis Mitogens
  • cytokine
  • cytokines
  • IFN Gamma
  • IFND
  • IL 1 beta
  • IL 10
  • IL 12 Receptor
  • IL 13
  • IL 17
  • IL 2
  • IL 4
  • IL 5
  • IL 6
  • IL 8
  • IL-1 Beta
  • IL-10
  • IL-12
  • IL-13
  • IL-17
  • IL-2
  • IL-2R
  • IL-4
  • IL-5
  • IL-6
  • IL-8
  • IL2 Receptor
  • Lymphocyte Blastogenesis Antigen
  • Lymphocyte Blastogenesis Mitogens
  • Mitogen Studies
  • Phytohemagglutinin
  • TNF Alpha
  • TNFa