Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation
Ordering Recommendation

Primarily for research and to support attempts to understand the pathogenesis of immune, infectious, allergic, or inflammatory disorders.

Cell Culture/Quantitative Multiplex Bead Assay
9-10 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
A control specimen needs to be sent with the patient specimen. The control specimen needs to be drawn from a normal, healthy individual who is not biologically related to the patient, and drawn at approximately the same time as and under similar conditions to the patient specimen. 
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control samples must be collected within 48 hours of test performance. 
Specimen Preparation
Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL whole blood (patient) AND 7 mL whole blood (control)) LIVE CELLS REQUIRED. Do not refrigerate or freeze.
Infant Minimum:
3 mL whole blood (patient) AND 7 mL whole blood (control). 
Storage/Transport Temperature
Unacceptable Conditions
Yellow (ACD Solution B). Specimens in transport longer than 48 hours.
New York State Clients: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable 
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data
Mitogens (Phytohemagglutinin, concanavalin A, pokeweed) are tested independently in lymphocyte culture. Peripheral Blood Mononuclear Cell (PBMC) cytokine responses to these mitogens are determined by quantitative multiplex bead assay. Results are used to understand the pathophysiology of immune, infectious, or inflammatory disorders, or may be used for research purposes.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

The following cytokines are reported: IL-2, sIL-2R (sCD25), IL-4, IL-5, IL-10, IL-13, IL-1b, IL-6, IL-17, TNF-a, and IFN-g. Results are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
Hotline History
CPT Code(s)
Component Test Code*Component Chart NameLOINC
2013110Cytokine Production Response, Mitogens
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • cytokine
  • Cytokine 12
  • cytokines
  • IFN
  • IFN G
  • IFN Gamma
  • IL 1
  • IL 1 B
  • IL 10
  • IL 13
  • IL 1B
  • IL 2
  • IL 2 R
  • IL 2R
  • IL 4
  • IL 5
  • IL 6
  • IL 8
  • IL-1 Beta
  • IL-10
  • IL-13
  • IL-2
  • IL-2R
  • IL-4
  • IL-5
  • IL-6
  • IL-8
  • IL1
  • IL1 B
  • IL1 Beta
  • IL10
  • IL13
  • IL2
  • IL2 R
  • IL2 Receptor
  • IL2R
  • IL4
  • IL5
  • IL6
  • IL8
  • TNF Alpha
  • Tumor Necrosis Factor alpha