Human Herpesvirus 8 (HHV-8) by Quantitative PCR
Ordering Recommendation

Detect and quantify herpesvirus 8 (HHV-8).

Quantitative Polymerase Chain Reaction
Mon, Thu
2-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Lavender (EDTA), Pink (K2 EDTA), or Serum Separator Tube (SST). 
Specimen Preparation
Separate serum or plasma from cells. Transport 1 mL plasma, serum, or whole blood in a sterile container. (Min: 0.5 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Heparinized specimens. 
Specimen source required. 
Ambient: 24 hours; Refrigerated: 1 week; Frozen: 1 year 
Reference Interval
Not detected
Interpretive Data
The quantitative range of this assay is 3.8-8.8 log copies/mL (6,670 - 667,000,000 copies/mL).

A negative result (less than 3.8 log copies/mL or less than 6,670 copies/mL) does not rule out the presence of PCR inhibitors in the patient specimen or HHV8 DNA concentrations below the level of detection of the test. Inhibition may also lead to underestimation of viral quantitation.

No international standard is currently available for calibration of this assay. Caution should be taken when interpreting results generated by different assay methodologies.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

The limit of quantification for this DNA test is 3.8 log copies/mL (6,670 copies/mL). If the test DID NOT DETECT the virus, the test result will be reported as "< 3.8 log copies/mL (< 6,670 copies/mL)." If the test DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."
Hotline History
Component Test Code*Component Chart NameLOINC
2013090HHV8 by Quantitative PCR, Source31208-2
2013091HHV8 by Quantitative PCR, Copy/mL49404-7
2013092HHV8 by Quantitative PCR, Log copy/mL
2013093HHV8 by Quantitative PCR, Interp39083-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
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