Patient Preparation

 

Collect

Lavender (EDTA) or pink (K₂EDTA). 

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 3 mL plasma into an ARUP Standard Transport Tube. (Min: 2 mL) Remove particulate material. This test requires a dedicated transport tube submitted only for HIV testing. 

Storage/Transport Temperature

Frozen. 

Unacceptable Conditions

Serum. Heparinized or citrated plasma specimens. Plasma preparation tube. Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens. 

Remarks

 

Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: 6 days; Frozen: 6 weeks (avoid repeated freeze/thaw cycles) 

Test Number	Components	Reference Interval
	HIV 1,2 Combo Antigen/Antibody	Negative
2012669	Human Immunodeficiency Virus Types 1 and 2 (HIV-1/2) Antibody Differentiation, Supplemental, with Reflex to HIV-1 Quantitative PCR	Test Number Components Reference Interval HIV-1 Antibody Negative HIV-2 Antibody Negative 0055598 Human Immunodeficiency Virus 1 by Quantitative PCR Not detected

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cells, Tissues, and Cellular and Tissue-Based Products (HCT/P).

The fourth-generation screen test is for the simultaneous qualitative detection of Human Immunodeficiency Virus Type 1 (HIV-1) p24 antigen and antibodies to HIV Type 1 (HIV-1 groups M and O) and HIV Type 2 (HIV-2). Results of the screen cannot be used to distinguish between the presence of HIV-1 p24 antigen, HIV-1 antibody, or HIV-2 antibody.

If the HIV-1,2 Combo Antigen/Antibody screen is repeatedly reactive, then the HIV-1/2 Ab Differentiation Immunoassay will be performed. Additional charges apply. The HIV-1/2 Ab Differentiation Immunoassay confirms and discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported.

If the HIV-1/2 Ab Differentiation Immunoassay is Negative or Indeterminate, then the Human Immunodeficiency Virus 1 by Quantitative PCR will be added. Additional charges apply.

This multi-test algorithm is recommended by the Centers for Disease Control and Prevention (CDC) and was adopted by the Clinical Laboratory Standards Institute (CLSI) for the diagnosis of HIV (refer to http://www.arupconsult.com/Topics/HIV.html).

Refer to the following tests for additional information regarding Performed or Reported times, Interpretive Data and Notes for the reflex tests of this panel:
Human Immunodeficiency Virus Types 1 and 2 (HIV-1/2) Antibody Differentiation, Supplemental with Reflex to HIV-1 Quantitative PCR (2012669);
Human Immunodeficiency Virus 1 by Quantitative PCR (0055598)

87389; if reflexed, add 86701 and 86702; if reflexed, add 87536

Component Test Code*	Component Chart Name	LOINC
2012675	HIV 1,2 Combo Antigen/Antibody	56888-1

* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

• Human Immunodeficiency Virus 1/O/2 (HIV-1/O/2) Antibodies, Preliminary Test With Confirmation
• 4th generation, Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody
• Fourth generation HIV
• HIV 1,2 Combo Antigen/Antibody
• HIV Diagnosis
• HIV-1/2 Antigen and Antibodies
• Human Immunodeficiency Virus 1/O/2 (HIV-1/O/2) Antigen/Antibody (Fourth Generation) Preliminary Test