Human Immunodeficiency Virus Types 1 and 2 (HIV-1/2) Antibody Differentiation, Supplemental, with Reflex to HIV-1 Quantitative NAAT, Plasma
Ordering Recommendation

Useful as a supplemental assay for repeatedly reactive third- or fourth-generation HIV screen tests, when following the CDC Proposed HIV Diagnostic Algorithm. This test cannot be used as a rapid screen nor as a follow-up for a positive rapid screen.

Qualitative Immunoassay/Quantitative Transcription-Mediated Amplification
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Lavender (EDTA), or Pink (K2EDTA). 
Specimen Preparation
Separate from cells within 24 hours of collection. Transfer 3 mL plasma into an ARUP Standard Transport Tube dedicated only for HIV testing. (Min: 1 mL) Remove particulate material. 
Storage/Transport Temperature
Unacceptable Conditions
Serum. Heparinized or citrated plasma specimens. Specimens submitted in plasma preparation tube. Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens. 
After separation from cells: Ambient: Unacceptable; Refrigerated:72 hours; Frozen: 3 months (avoid repeated freeze/thaw cycles) 
Reference Interval
Effective November 12, 2018
Test Number
Reference Interval
HIV-1 AntibodyNegative
HIV-2 AntibodyNegative
3000867Human Immunodeficiency Virus 1 (HIV-1) by Quantitative NAAT, PlasmaNot detected

Interpretive Data
This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cells, Tissues, and Cellular and Tissue-Based Products (HCT/P).

For use only when patient has a repeatedly reactive third or fourth generation HIV screen test result. This test discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported. This test is for use as the antibody differentiation test in the specific multi-test algorithm. It is not to be ordered as a rapid screen test and cannot be used as a supplemental test if the initial screen test was a rapid test.

If the HIV-1/ 2 Antibody Differentiation Immunoassay is Negative or Indeterminate, then the Human Immunodeficiency Virus 1 (HIV-1) by Quantitative NAAT, Plasma, will be added. Additional charges apply. Refer to Human Immunodeficiency Virus 1 (HIV-1) by Quantitative NAAT, Plasma (ARUP test code 3000867) for additional information regarding Performed or Reported times, Interpretive Data and Notes for the reflex test.

The multi-test algorithm is recommended by the Centers for Disease Control and Prevention (CDC) and the Clinical Laboratory Standards Institute (CLSI) for the diagnosis of HIV (refer to
Hotline History
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CPT Code(s)
86701; 86702; if reflexed, add 87536
Component Test Code*Component Chart NameLOINC
2012670HIV-1/2 Ab Differentiation Immunoassay8251-1
2012671HIV-1 Antibody68961-2
2012672HIV-2 Antibody81641-3
2012673HIV Serologic Interpretation80203-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Geenius
  • HIV Diagnosis
  • HIV Multispot
  • HIV-1 & 2 Antibody Differentiation
  • HIV-1 and HIV-2 Antibody Differentiation
  • HIV-1 Antibody by Multispot
  • Human Immunodeficiency Virus 1/2 (HIV-1/2) Antibody, Differentiation With Reflex to HIV-1 RNA