Human Immunodeficiency Virus Types 1 and 2 (HIV-1/2) Antibody Differentiation, Supplemental, with Reflex to HIV-1 Quantitative PCR
Ordering Recommendation

Useful as a supplemental assay for repeatedly reactive third- or fourth-generation HIV screen tests, when following the CDC Proposed HIV Diagnostic Algorithm. This test cannot be used as a rapid screen nor as a follow-up for a positive rapid screen.

Qualitative Immunoassay/Quantitative Polymerase Chain Reaction
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Lavender (EDTA), or pink (K2EDTA). 
Specimen Preparation
Separate plasma from cells ASAP or within 2 hours of collection. Transfer 3 mL plasma into an ARUP Standard Transport Tube dedicated only for HIV testing. (Min: 1.5 mL) Remove particulate material. 
Storage/Transport Temperature
Unacceptable Conditions
Serum. Heparinized or citrated plasma specimens. Specimens submitted in plasma preparation tube. Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens. 
This test requires a dedicated tube of EDTA plasma submitted only for HIV testing. 
After separation from cells: Ambient: Unacceptable; Refrigerated: 6 days; Frozen: 6 weeks (avoid repeated freeze/thaw cycles) 
Reference Interval
Test NumberComponentsReference Interval
HIV-1 AntibodyNegative
HIV-2 AntibodyNegative
0055598Human Immunodeficiency Virus 1 by Quantitative PCRNot detected

Interpretive Data
This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cells, Tissues, and Cellular and Tissue-Based Products (HCT/P).

For use only when patient has a repeatedly reactive third or fourth generation HIV screen test result. This test discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported. This test is for use as the antibody differentiation test in the specific multi-test algorithm. It is not to be ordered as a rapid screen test and cannot be used as a supplemental test if the initial screen test was a rapid test.

If the HIV-1/ 2 Antibody Differentiation Immunoassay is Negative or Indeterminate, then the Human Immunodeficiency Virus 1 by Quantitative PCR will be added. Additional charges apply. Refer to Human Immunodeficiency Virus 1 by Quantitative PCR (0055598) for additional information regarding Performed or Reported times, Interpretive Data and Notes for the reflex test.

The multi-test algorithm is recommended by the Centers for Disease Control and Prevention (CDC) and the Clinical Laboratory Standards Institute (CLSI) for the diagnosis of HIV (refer to
Hotline History
CPT Code(s)
86701; 86702; if reflexed, add 87536
Component Test Code*Component Chart NameLOINC
2012670HIV-1/2 Ab Differentiation Immunoassay8251-1
2012671HIV-1 Antibody29893-5
2012672HIV-2 Antibody30361-0
2012673HIV Serologic Interpretation80203-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Geenius
  • HIV Diagnosis
  • HIV Multispot
  • HIV-1 & 2 Antibody Differentiation
  • HIV-1 and HIV-2 Antibody Differentiation
  • HIV-1 Antibody by Multispot
  • Human Immunodeficiency Virus 1/2 (HIV-1/2) Antibody, Differentiation With Reflex to HIV-1 RNA