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Human Immunodeficiency Virus Types 1 and 2 (HIV-1/2) Antibody Differentiation, Supplemental, with Reflex to HIV-1 Quantitative PCR
2012669
Ordering Recommendation

Useful as a supplemental assay for repeatedly reactive third- or fourth-generation HIV screen tests, when following the CDC Proposed HIV Diagnostic Algorithm. This test cannot be used as a rapid screen nor as a follow-up for a positive rapid screen.

Mnemonic
HIV AB DIF
Methodology
Qualitative Immunoassay/Quantitative Polymerase Chain Reaction
Performed
Varies
Reported
1-2 days
N/A
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), or pink (K2EDTA). 
Specimen Preparation
Separate plasma from cells ASAP or within 2 hours of collection. Transfer 3 mL plasma into an ARUP Standard Transport Tube dedicated only for HIV testing. (Min: 1.5 mL) Remove particulate material. 
Storage/Transport Temperature
Frozen. 
Unacceptable Conditions
Serum. Heparinized or citrated plasma specimens. Specimens submitted in plasma preparation tube. Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens. 
Remarks
This test requires a dedicated tube of EDTA plasma submitted only for HIV testing. 
Stability
After separation from cells: Ambient: Unacceptable; Refrigerated: 6 days; Frozen: 6 weeks (avoid repeated freeze/thaw cycles) 
Reference Interval
Test NumberComponentsReference Interval
HIV-1 AntibodyNegative
HIV-2 AntibodyNegative
0055598Human Immunodeficiency Virus 1 by Quantitative PCRNot detected

Interpretive Data
This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cells, Tissues, and Cellular and Tissue-Based Products (HCT/P).

Note
For use only when patient has a repeatedly reactive third or fourth generation HIV screen test result. This test discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported. This test is for use as the antibody differentiation test in the specific multi-test algorithm. It is not to be ordered as a rapid screen test and cannot be used as a supplemental test if the initial screen test was a rapid test.

If the HIV-1/ 2 Antibody Differentiation Immunoassay is Negative or Indeterminate, then the Human Immunodeficiency Virus 1 by Quantitative PCR will be added. Additional charges apply. Refer to Human Immunodeficiency Virus 1 by Quantitative PCR (0055598) for additional information regarding Performed or Reported times, Interpretive Data and Notes for the reflex test.

The multi-test algorithm is recommended by the Centers for Disease Control and Prevention (CDC) and the Clinical Laboratory Standards Institute (CLSI) for the diagnosis of HIV (refer to http://www.arupconsult.com/Topics/HIV.html).
CPT Code(s)
86701; 86702; if reflexed, add 87536
Components
Component Test Code*Component Chart NameLOINC
2012670HIV-1/2 Ab Differentiation Immunoassay8251-1
2012671HIV-1 Antibody29893-5
2012672HIV-2 Antibody30361-0
2012673HIV Serologic Interpretation80203-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Geenius
  • HIV Diagnosis
  • HIV Multispot
  • HIV-1 & 2 Antibody Differentiation
  • HIV-1 and HIV-2 Antibody Differentiation
  • HIV-1 Antibody by Multispot
  • Human Immunodeficiency Virus 1/2 (HIV-1/2) Antibody, Differentiation With Reflex to HIV-1 RNA