Feedback
RET-CCDC6 and RET-NCOA4 (RET-PTC1 and RET-PTC3) Translocations Detection by PCR
2012605
Ordering Recommendation

Detects RET-CCDC6 and RET-NCOA4 gene fusions associated with thyroid cancer.

Mnemonic
RET PTC
Methodology
Reverse Transcription Polymerase Chain Reaction (RT-PCR)
Performed
RNA Isolation: Sun-Sat
Assay: Mon, Thu
Reported
10-12 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Tumor tissue. 
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides) Tissue block will be returned after testing.
A Fine Needle Aspirate (FNA) smear may also be submitted. Prepare FNA smear with Diff-Quik or equivalent stain by standard methods (air-dried slides are preferred). Number of slides needed is dependent on the tumor cellularity of the smear. (Min: 1 slide). Slide(s) will be destroyed during testing process and will not be returned to client.
Transport of a block and/or slide(s) in a tissue transport kit is preferred (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Also acceptable: FNA specimens stored in saline, PreservCyt or CytoLyt. 
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months. 
Unacceptable Conditions
Less than 10 percent tumor. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives. Decalcified specimens. FNA smears with less than 50 tumor cells. 
Remarks
 
Stability
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
FNA in Saline: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable 
Reference Interval
Interpretive Data
Refer to report.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
CPT Code(s)
81479; add 88381 for FFPE
Components
Component Test Code*Component Chart NameLOINC
2002148Block ID57723-9
2012606RET-PTC Translocation Result
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases