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HLA-B by Next Generation Sequencing
2012486
Ordering Recommendation

The intended use of this test is pre-transplant allele matching. Do not use for HLA-related disease screening or diagnosis (eg, celiac disease, narcolepsy, rheumatologic diseases).

Mnemonic
HLA B NGS
Methodology
Polymerase Chain Reaction/Massive Parallel Sequencing
Performed
Varies
Reported
10-13 days
N/A
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA). Also acceptable: yellow (ACD Solution A). 
Specimen Preparation
Transport 4 mL whole blood in the original container. (Min: 1 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Yellow (ACD Solution B) specimens. Clotted, grossly hemolyzed, or heparinized specimens. 
Remarks
 
Stability
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
By Report
Interpretive Data


Components
Component Test Code*Component Chart NameLOINC
2012487HLA-B, Class I - Locus B*, Allele 113299-3
2012488HLA-B, Class I - Locus B*, Allele 213299-3
2012489HLA-B by NGS, Interpretation45028-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases

Performed at Children’s Hospital of Philadelphia