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SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
2012074
Ordering Recommendation

Order as secondary screen based on results of ANA test or if ANA IFA is negative and Sjögren syndrome, systemic lupus erythematosus (SLE), systemic sclerosis, or myositis is strongly suspected.

Mnemonic
SSA RO
Methodology
Semi-Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube (SST). 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Test NumberComponentsReference Interval
SSA 52 (Ro) (ENA) Antibody, IgG29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA 60 (Ro) (ENA) Antibody, IgG29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Interpretive Data
SSA 52 (Ro) and/or SSA 60 (Ro) antibodies are associated with a diagnosis of Sjögren syndrome, systemic lupus erythematosus (SLE), and systemic sclerosis. SSA-52 antibody overlaps significantly with the major SSc-related antibodies. SSA-52 antibody occurs frequently in patients with inflammatory myopathies, often in the presence of interstitial lung disease.

Components
Component Test Code*Component Chart NameLOINC
0050691SSA 52 (Ro) (ENA) Antibody, IgG31625-7
2012055SSA 60 (Ro) (ENA) Antibody, IgG53019-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Anti-Ro
  • Anti-SSA; Anti-SSA (Ro) (ENA) Antibody
  • Ro
  • Ro Autoantibodies
  • Sjogren Syndrome
  • Sjogren's Antibody
  • SSA Autoantibodies