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HIV-1 Genotyping and Tropism by Next Generation Sequencing (DEEPGEN)
2011279
Ordering Recommendation

HIV-1 deep sequencing test predicts susceptibility to
• Protease inhibitors (PI)
• Reverse transcriptase inhibitors (NRTI, NNRTI)
• Integrase inhibitors (INI)
• Receptor inhibitors (CCR5 or CXCR4 tropism)

Some studies have shown improved outcomes using deep versus traditional (Sanger) HIV sequencing, but other studies have not.
• 2014 European case-control study for NNRTIs (refer to PubMed ID: 25336166)
• 2015 French study on highly-pretreated patients (refer to PubMed ID: 25755001)
• 2016 Uganda retrospective study on antiviral treatment failures (refer to PubMed ID: 27001818) 

Mnemonic
HIV DEEPGN
Methodology
Massively Parallel Sequencing
Performed
Varies
Reported
15-18 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA). 
Specimen Preparation
Specimens must be separated from cells within 6 hours of collection. Transfer 5 mL plasma to ARUP Standard Transport Tubes and freeze immediately. (Min: 1 mL) 
Storage/Transport Temperature
Critical Frozen: Separate specimens must be submitted with multiple tests are ordered. 
Unacceptable Conditions
 
Remarks
 
Stability
Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: Indefinitely 
Reference Interval
By Report
Interpretive Data


Note
Provide patient's most recent viral load with date of collection. This test should be used for patients with documented HIV-1 infection and a viral load greater than 1000 copies/mL.
Components
Component Test Code*Component Chart NameLOINC
2011280DEEPGEN Viral Load
2011281DEEPGEN Viral Load Date
2011282EER HIV-1 Genotyping and Tropism by NGS11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases

Performed at University Hospital Translational Lab (UHTL)