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HLA Class II Panel (DRB1 and DQB1) by Next Generation Sequencing
2011272
Ordering Recommendation

Test is not intended for HLA-related disease screening or diagnosis (eg, celiac disease, narcolepsy, rheumatologic diseases).

Mnemonic
HLA II NGS
Methodology
Polymerase Chain Reaction/Massively Parallel Sequencing
Performed
Varies
Reported
8-15 days
N/A
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA).  Also acceptable: Yellow (ACD Solution A). 
Specimen Preparation
Transfer 4 mL whole blood to an ARUP Standard Transport Tube. (Min: 1 mL). 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Yellow (ACD Soultion B) specimens.  Clotted, grossly hemolyzed, or heparinized specimens. 
Remarks
 
Stability
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data
Purpose: To identify HLA-DRB1 and DQB1 allelic polymorphisms on specimens for transplant candidates and their donors.
Methodology: PCR followed by next generation sequencing of HLA-DRB1 and DQB1 loci.
Analytical Sensitivity & Specificity:>99 percent.
Limitations: Rare diagnostic errors can occur due to primer site mutations.
Test Results: Results are reported as HLA locus (DRB1, or DQB1)* followed by the four digit assigned allele.

Note
CPT Code(s)
81382 x2
Components
Component Test Code*Component Chart NameLOINC
2011273HLA Class II - Locus DQB1*, Allele 1
2011274HLA Class II - Locus DQB1*, Allele 2
2011275HLA Class II - Locus DRB1*, Allele 1
2011276HLA Class II - Locus DRB1*, Allele 2
2011277HLA Class II Sequencing, Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HLA-DQB1
  • HLA-DRB1
  • Human leukocyte antigen

Performed at Children’s Hospital of Philadelphia