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HLA Class I Panel (ABC) by Next Generation Sequencing
2011264
Ordering Recommendation

Test is intended for pre-transplant allele matching. Do not use for specific disease screening or diagnosis (eg, celiac disease, rheumatologic diseases).

Mnemonic
HLA I NGS
Methodology
Polymerase Chain Reaction/Massively Parallel Sequencing
Performed
Varies
Reported
8-15 days
N/A
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA).  Also acceptable Yellow (ACD Solution A) 
Specimen Preparation
Transfer 4 mL whole blood to an ARUP Standard Transport Tube. (Min: 1 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Yellow (ACD Solution B) specimens.  Clotted, grossly hemolyzed, or heparinized specimens. 
Remarks
 
Stability
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data
Purpose: To identify HLA-A,-B, and-C allelic polymorphisms on specimens for transplant candidates and their donors.
Methodology: PCR followed by next generation sequencing of HLA-A,-B and-C loci.
Analytical Sensitivity & Specificity:>99 percent.
Limitations: Rare diagnostic errors can occur due to primer site mutations.
Test Results: Results are reported as HLA locus (A, B, or C)* followed by the four digit assigned allele.

Note
CPT Code(s)
81379
Components
Component Test Code*Component Chart NameLOINC
2011265HLA Class I - Locus B*, Allele 113299-3
2011266HLA Class I - Locus B*, Allele 213299-3
2011267HLA Class I - Locus A*, Allele 138548-4
2011268HLA Class I - Locus A*, Allele 238548-4
2011269HLA Class I - Locus C*, Allele 142663-5
2011270HLA Class I - Locus C*, Allele 242663-5
2011271HLA-ABC Sequencing, Interpretation53767-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HLA-A
  • HLA-B
  • HLA-C
  • Human leukocyte antigen

Performed at Children’s Hospital of Philadelphia