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Regulatory T-Cell Panel
2010172
Ordering Recommendation

May be useful when evaluating patients for a rare primary immune deficiency called IPEX (immune dysregulation, polyendocrinopathy, enteropathy, X-linked), graft-vs-host disease in post-hematopoietic stem cell transplantation, some malignancies, or autoimmune diseases.

Mnemonic
TREGS
Methodology
Quantitative Flow Cytometry
Performed
Sun-Sat
Reported
1-3 days
1-3 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or green (sodium heparin). 
Specimen Preparation
Transport 4 mL whole blood. (Min: 0.5 mL) 
Storage/Transport Temperature
Refrigerated. Also acceptable: Room temperature.  
Unacceptable Conditions
Specimens older than 72 hours. Clotted or hemolyzed specimens. 
Remarks
Specimens must be analyzed within 72 hours of collection. 
Stability
Ambient: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable 
Reference Interval
Available SeparatelyComponentsReference Interval (Adults 19 & older)
NoPercent CD4+CD25+CD127Low (Tregs)4.0-10.7 percent of CD4+
NoAbs CD4+CD25+CD127Low (Tregs)20.0-126.3 cells/µL
NoPercent CD4+CD25+CD127LowCD45RA+ (Nn Tregs)0.4-4.5 percent of CD4+
NoAbs CD4+CD25+CD127LowCD45RA+ (Nn Tregs)2.3-54.0 cells/µL
NoPercent CD4+CD25+CD127LowCD45RO+ (N Tregs)2.4-8.7 percent of CD4+
NoAbs CD4+CD25+CD127LowCD45RO+ (N Tregs)14.3-88.3 cells/µL

Interpretive Data
Regulatory T-cells (Tregs) are a population of CD4+, CD25+, CD127Low T-cells that suppress aberrant immune responses. CD25 is also expressed on activated T-cells; however, CD127Low expression on Tregs differentiates them from activated T-cells. Natural (N) Tregs express the memory marker CD45RO and are characterized by a limited ability to proliferate. Natural Naive (Nn) Tregs express the naive marker CD45RA, are more prominent in young adults, and have high proliferation capacity. Reduced Tregs may predispose to autoimmune disease. Absence of Tregs due to mutations in FOXP3 cause a rare primary immune deficiency called IPEX (immune dysregulation, polyendocrinopathy, enteropathy, X-linked). Increased Tregs in donor stem cell allografts are associated with reduced occurrence of graft versus host disease after stem cell transplantation. Increased Tregs are also associated with some malignancies.

Compliance Statement A: Analyte specific reagents (ASR) are used in many laboratory tests necessary for standard medical care and generally do not require U.S. Food and Drug Administration (FDA) approval or clearance. This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. This test should not be regarded as investigational or for research use.

Components
Component Test Code*Component Chart NameLOINC
2010173% CD4+CD25+CD127Low (Tregs)
2010174Abs CD4+CD25+CD127Low (Tregs)
2010175% CD4+CD25+CD127LowCD45RA+ (Nn Tregs)
2010176Abs CD4+CD25+CD127LowCD45RA+ (Nn Tregs)
2010177% CD4+CD25+CD127LowCD45RO+ (N Tregs)
2010178Abs CD4+CD25+CD127LowCD45RO+ (N Tregs)
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Regulatory T-cells
  • TREGS