Influenza A Virus H1/H3 Subtyping by PCR with Reflex to  H1N1 (2009) Oseltamivir Resistance by Sequencing
Ordering Recommendation

Detect and subtype influenza A virus (H1/H3). Determine H1N1 oseltamivir (Tamiflu) drug resistance.

Qualitative Reverse Transcription Polymerase Chain Reaction/Pyrosequencing
3-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Respiratory specimen: Bronchoalveolar lavage (BAL), nasal wash, nasopharyngeal swab, or pleural fluid. 
Specimen Preparation
Fluid: Transfer 2 mL respiratory specimen to a sterile container. (Min: 1 mL) Also acceptable: Transfer to viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swabs: Place in viral transport media. Place each specimen in an individually sealed bag. 
Storage/Transport Temperature
Unacceptable Conditions
Dry swabs. 
Specimen source required. 
Ambient: 8 hours; Refrigerated: 5 days; Frozen: 2 months 
Reference Interval
Interpretive Data
This test targets the H3 and 2009-H1 hemagglutinin genes. The current circulating Influenza A strains are detected and typed (H1N1 and H3N2), however, other H1 and H3 subtypes may also be detected.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

If Influenza A Virus H1/H3 Subtyping by PCR result is detected for H1N1, then H1N1 (2009) Oseltamivir Resistance by Sequencing will be added.  Additional charges apply.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
2007470Influenza A Virus Subtyping Source
2007471Influenza A Virus Subtyping by RT-PCR34487-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.