Celiac Disease Reflexive Cascade
Ordering Recommendation

Preferred reflex screening test for celiac disease. May aid in monitoring adherence to gluten-free diet.

Quantitative Nephelometry/Semi-Quantitative Enzyme-Linked Immunosorbent Assay//Semi-Quantitative Indirect Fluorescent Antibody
2-6 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Plasma. Contaminated, hemolyzed, grossly icteric or grossly lipemic specimens.  
After separation from cells: Ambient: 8 hours; Refrigerated: 8 days; Frozen: 1 year (if frozen within 24 hours)  
Reference Interval
Effective February 16, 2016
Test Number
Reference Interval
0050340Immunoglobulin AEffective February 16, 2016
0-30 days: 1-7 mg/dL
1 month: 1-53 mg/dL
2 months: 3-47 mg/dL
3 months: 5-46 mg/dL
4 months: 4-72 mg/dL
5 months: 8-83 mg/dL
6 months: 8-67 mg/dL
7-8 months: 11-89 mg/dL
9-11 months: 16-83 mg/dL
1 year: 14-105 mg/dL
2 years: 14-122 mg/dL
3 years: 22-157 mg/dL
4 years: 25-152 mg/dL
5-7 years: 33-200 mg/dL
8-9 years: 45-234 mg/dL
10 years and older: 68-408 mg/dL

0051689Celiac Disease Dual Antigen Screen19 Units or less: Negative - No significant level of detectable IgA or IgG antibodies against human tissue transglutaminase or gliadin peptide.

20 Units or greater: Positive - Presence of IgA and/or IgG antibodies against human tissue transglutaminase and/or gliadin peptide; suggests possibility of certain gluten sensitive enteropathies such as celiac disease and dermatitis herpetiformis.
0051357Deamidated Gliadin Peptide (DGP) Antibody, IgA19 Units or less: Negative
20-30 Units: Weak Positive
31 Units or greater: Positive
0051359Deamidated Gliadin Peptide (DGP) Antibody, IgG19 Units or less: Negative
20-30 Units: Weak Positive
31 Units or greater: Positive
0097709Tissue Transglutaminase (tTG) Antibody, IgA3 U/mL or less: Negative
4-10 U/mL: Weak Positive
11 U/mL or greater: Positive
0050736Endomysial Antibody, IgA by IFALess than 1:10
0056009Tissue Transglutaminase Antibody, IgG5 U/mL or less: Negative
6-9 U/mL: Weak Positive
10 U/mL or greater: Positive

Interpretive Data
Refer to report.

The Celiac Disease Reflexive Cascade begins with Immunoglobulin A. Depending on findings, one or more reflexive tests may be required in order to provide a clinical interpretation. Tests added may include Tissue Transglutaminase Antibody, IgA; Tissue Transglutaminase Antibody, IgG; Endomysial Antibody, IgA by IFA; Deamidated Gliadin Peptide (DGP) Antibody, IgA; Deamidated Glaidin Peptide (DGP) Antibody, IgG; and/or Celiac Disease Dual Antigen Screen. Refer to the Celiac Testing Algorithm found at Additional charges apply.
CPT Code(s)
82784; if reflexed additional CPT codes may apply: 83516, 83516 x2 and/or 86256.
add 83516; if reflexed to tTG IgA and Gliadin IgA, add 83516 x 2; if reflexed to tTG IgG and Gliadin IgG, add 83516 x2.; if IgA is greater than or equal to the normal age range, add 83516; if reflexed, add 86256 and 83516.
Component Test Code*Component Chart NameLOINC
0050340Immunoglobulin A2458-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.