ERBB2 (HER2/neu) (HercepTest) by Immunohistochemistry, Tissue with Reflex to Dual ISH if 2+ (INACTIVE as of  05/18/15: Refer to 0049178)
Ordering Recommendation
1-5 days
Reflexed to HER2Dual ISH (2007410) if 2+, Dual ISH will be performed and reported within 3-5 days if no repeats are required.
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Immunohistochemistry Stain Form Recommended  (ARUP form #32978)Immunohistochemistry Stain Form Recommended (ARUP form #32978)
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Tumor tissue. 
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue within 30 minutes of removal from patient. Fixative duration: 8-48 hours. Transport tissue block or 10 unstained (3-to 5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800)522-2787. (Min: 6 slides) If sending precut slides, do not oven bake. 
Storage/Transport Temperature
Room temperature or refrigerated. Ship in cooled container during summer months. 
Unacceptable Conditions
Paraffin block with no tumor tissue remaining. Specimens with fixation delayed for more than 30 minutes. Specimens fixed in any other fixative other than 10 percent neutral buffered formalin. Tissue fixed for less than 8 hours or greater than 48 hours. Cytology specimens fixed in alcohol. 
Pathology report including tissue source and tumor origin must be submitted. Document time from tissue acquisition to fixation and fixation duration on requisition or enter at time of order. IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry form (#32978) with an ARUP client number. For additional technical details, please contact ARUP Client Services at (800) 522-2787. 
Ambient: Indefinite; Refrigerated: Indefinite; Frozen: Unacceptable 
Reference Interval
Interpretive Data
Refer to report.

If ERBB2 (HercepTest) result is 2+, then ERBB2 (HER2/neu) Gene Amplification by Dual In Situ Hybridization will be added. Additional charges apply.
CPT Code(s)
88360, if reflexed, add 88377
Component Test Code*Component Chart NameLOINC
0049171Homogenous Pattern
0049181Hercep Comments
0049184Intensity of Staining
0049188Percent of Cells/Circumferential
2007786ERBB2 (HercepTest) Rflx to DISH if 2+
2009596Homogeneous dark circumferential pattern
2009597Uniformity of staining
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • c-erbB2 Hercept IHC, 2+ Dual ISH reflex
  • ERBB2 Hercept IHC, 2+ Dual ISH reflex
  • HER-2/neu Hercept IHC, 2+ Dual ISH reflex
  • HER2 (ERBB2 (HercepTest{R}) Tissue Assay, Paraffin) with Reflex to Dual ISH if 2+
  • HER2 IHC (ERBB2 (HercepTest{R}) Tissue Assay, Paraffin)
  • Herceptin (ERBB2 (HercepTest{R}) Tissue Assay, Paraffin)
  • INFORM Dual In-situ Hybridization Probe reflex