Tricyclic Antidepressants, Quantitative, Serum or Plasma
Ordering Recommendation

Optimize drug therapy and monitor patient adherence.

Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Mon, Wed, Fri
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Timing of specimen collection: Pre-dose (trough) draw - At steady-state concentration. 
Plain red. Also acceptable: Lavender (K2 or K3EDTA) or pink (K2EDTA). 
Specimen Preparation
Separate serum or plasma from cells within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Whole blood. Gel separator tubes, light blue (citrate), or yellow (SPS or ACD solution). 
After separation from cells: Ambient: 5 days; Refrigerated: 2 weeks; Frozen: 6 months 
Reference Interval
Effective November 18th, 2013
Therapeutic Range
Amitriptyline (Elavil, Vanatrip)Not establishedNot established
Nortriptyline (Aventyl, Pamelor)50-150 ng/mLGreater than 500 ng/mL
Total Amitriptyline + Nortriptyline95-250 ng/mLGreater than 500 ng/mL
Imipramine (Tofranil)Not establishedNot established
Desipramine (Norpramin)100-300 ng/mLGreater than 500 ng/mL
Total Imipramine + Desipramine150-300 ng/mLGreater than 500 ng/mL
Doxepin (Sinequan, Zonalon)Not establishedNot established
NordoxepinNot establishedNot established
Total Doxepin + Nordoxepin100-300 ng/mLGreater than 500 ng/mL
Protriptyline (Vivactil)70-240 ng/mLGreater than 400 ng/mL
Clomipramine (Anafranil)Not establishedNot established
NorclomipramineNot establishedNot established
Total Clomipramine + Norclomipramine220-500 ng/mLGreater than 900 ng/mL

Interpretive Data

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

This test is used to quantitate the following tricyclic antidepressants: amitriptyline, clomipramine, desipramine, doxepin, imipramine, norclomipramine, nordoxepin, nortriptyline, and protriptyline.
Hotline History
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0090020Amitriptyline/Nortriptyline, Total, SP3335-7
0090024Amitriptyline, SP3333-2
0090065Desipramine, SP3531-1
0090100Doxepin, SP3579-0
0090101Doxepin/Nordoxepin Total, SP3582-4
0090105Nordoxepin, SP3862-0
0090106Protriptyline, SP3999-0
0090135Imipramine/Desipramine Total, SP9627-1
0090139Imipramine, SP3690-5
0090210Nortriptyline, SP3872-9
0099320Clomipramine/Norclomipramine, Total, SP3493-4
0099335Norclomipramine, SP3536-0
0099437Clomipramine (Anafranil), SP3491-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Tricyclic Antidepressant Screen
  • Tricyclic Screen