ALK (D5F3) with Interpretation by Immunohistochemistry
Ordering Recommendation

Determine eligibility for tyrosine kinase inhibitor (TKI) therapy, primarily in individuals with pulmonary adenocarcinomas. Detects ALK fusion proteins.

1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Immunohistochemistry Stain Form Recommended  (ARUP form #32978)Immunohistochemistry Stain Form Recommended (ARUP form #32978)
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect or contact ARUP Client Services at (800) 522-2787. (Min. 3 slides). If sending precut slides, do not oven bake.  
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Paraffin block with no tumor tissue remaining; specimens fixed in any fixative other than 10 percent neutral buffered formalin.  
This test code includes pathologist interpretation. Include surgical pathology report. IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. For additional technical details, please contact ARUP Client Services at (800) 522-2787.  
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
This test includes pathologist interpretation of results. Include surgical pathology report with specimen submitted for testing. Indicate tissue site on requisition.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
2007325ALK(D5F3) by IHC Reference Number57723-9
2007327ALK(D5F3) by IHC Result35474-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • ALK D5F3 antibody staining
  • ALK D5F3 staining
  • ALK rearrangement by IHC
  • ALK rearrangement staining