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HLA-B Genotype
2006986
Ordering Recommendation

May be useful in immunization/vaccination trials or may aid in the clinical diagnosis of diseases strongly associated with the HLA-B locus.

Mnemonic
HLA B
Methodology
Polymerase Chain Reaction/Sequence Specific Oligonucleotide Probe Hybridization
Performed
Mon-Fri
Reported
3-7 days
N/A
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). 
Specimen Preparation
Transport 5 mL whole blood. (Min: 3 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Specimens collected in green (sodium or lithium heparin) tubes. 
Remarks
 
Stability
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data
Background Information for HLA-B Genotype:
Purpose:
For immunization/vaccination trials or to aid the clinical diagnosis of diseases strongly associated with the HLA-B locus.
Methodology:
PCR followed by Sequence Specific Oligonucleotide Probe Hybridization of HLA-B locus.
Analytical Sensitivity & Specificity:
Medium to high resolution of HLA-B locus.
Limitations:
The presence of a disease-associated HLA combination does not establish a diagnosis. If fewer than 2 alleles are reported for a locus, the patient is likely homozygous. Rare diagnostic errors can occur due to primer or probe site mutations. This test is not sufficient for comprehensive HLA evaluation for clinical hematopoietic stem cell transplantation; for pre-transplant allele matching, consider HLA Class I (ABC) by Next Generation Sequencing-(ARUP test code 2011264) and/or HLA Class II (DRB1 and DQB1) by Next Generation Sequencing (ARUP test code 2011272).

Occasionally the specific allele cannot be determined; in this case, the most likely allele assignment is made followed by a sequence of letters indicating other possible allele assignments. Interpretation of allele codes can be found at http://bioinformatics.nmdp.org/HLA/alpha.v3.html.

Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.

Compliance Statement C: The performance characteristics of this test were validated by ARUP Laboratories. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test. However, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. ARUP is authorized under Clinical Laboratory Improvement Amendments (CLIA) and by all states to perform high-complexity testing. Counseling and informed consent are recommended for genetic testing. Consent forms are available online.

Note
Order this test for single antigen HLA-B identification. Please specify antigens.
CPT Code(s)
81380
Components
Component Test Code*Component Chart NameLOINC
2002785HLA Class I, Locus B*, Allele 113299-3
2002786HLA Class I, Locus B*, Allele 213299-3
2006987HLA-B Genotype Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases

Performed at UUHC - H&I Lab