Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by ELISA, with Reflex to HIV-1 Antibody Confirmation by Western Blot
Ordering Recommendation
Qualitative Enzyme-Linked Immunosorbent Assay/Qualitative Western Blot
Mon, Wed-Sat
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube. Also acceptable: Lavender (EDTA), pink (K2EDTA), green (sodium or lithium heparin), or red (clot activator) 
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. Remove particulate material. (Min: 0.5 mL). 
Storage/Transport Temperature
Unacceptable Conditions
Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens. 
After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles) 
Reference Interval
Test NumberComponentsReference Interval
Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by ELISANegative
0020284Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western BlotNegative

Interpretive Data
This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

This fourth-generation screen test is for the simultaneous qualitative detection of Human Immunodeficiency Virus Type 1 (HIV-1) p24 antigen and antibodies to HIV Type 1 (HIV-1 groups M and O) and HIV Type 2 (HIV-2), Results of the screen cannot be used to distinguish between the presence of HIV-1 p24 antigen, HIV-1 antibody, or HIV-2 antibody.

If the HIV-1,2 Combo Antigen/Antibodies screen is repeatedly reactive, then the Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot will be added. Additional charges apply.

Performed and Reported times indicated are for the screening portion of this test. Refer to Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot (ARUP test code 0020284) for additional information regarding Performed or Reported times for the reflex portion of this test.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
2006611HIV-1,2 Combo Antigen/Antibody56888-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • 4th generation HIV screen; HIV Ag/Ab combination screen
  • Fourth generation HIV
  • HIV 1,2 Combo Antigen/Antibody
  • HIV Diagnosis
  • HIV-1,2 Combo Antigen/Antibody