IDH1 and IDH2 Mutation Analysis, exon 4
2006444
Ordering Recommendation
Secondary test for prognostication of CN-AML.
Submit With Order
Mnemonic
IDH1-2
Methodology
Polymerase Chain Reaction/Sequencing
Performed
Varies
Reported
12-14 days
N/A
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) OR bone marrow (EDTA) OR tumor tissue.  
Specimen Preparation
Whole blood: Transport 5 mL (Min: 1 mL) OR Bone marrow: Transport 3 mL (Min: 1 mL) OR FFPE tumor tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 4 unstained 5-micron thick slides (Min: 3 slides). Transport block and/or slid (s) in a tissue transport kit (ARUP supply # 47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800)522-2787.  
Storage/Transport Temperature
Whole blood OR Bone marrow: Refrigerated. FFPE tumor tissue: Room temperature or refrigerated. Ship in cooled containers during summer months.  
Unacceptable Conditions
Whole blood OR Bone marrow: Serum or plasma. Specimens collected in anticoagulants other than EDTA. Frozen specimens. Clotted or grossly hemolyzed specimens. FFPE tumor tissue: No tumor in tissue. Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Decalcified specimens.  
Remarks
For FFPE specimens include surgical pathology report. Tissue block will be returned after testing.  
Stability
Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
FFPE tumor tissue:
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report.

See Compliance Statement B: www.aruplab.com/CS
Note
CPT Code(s)
81403 x2; FFPE tumor tissue, add 88381
Components
Component Test Code*Component Chart Name
2006445IDH1 and IDH2 Mutation Results
2006510IDH 1-2 Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • IDH1
  • IDH1 SNP
  • IDH2
  • Isocitrate dehydrogenase (IDH) 1 and 2
  • Isocitrate Dehydrogenase 1
  • Isocitrate Dehydrogenase 2
  • NADP+