Bilirubin, CSF
Ordering Recommendation

Useful as an indicator of in vivo breakdown of hemoglobin to differentiate cerebral hemorrhage from a traumatic tap.

Quantitative Spectrophotometry
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
CSF. Collect at least 12 hours after suspected hemorrhage. Use the last (ideally the fourth) specimen post lumbar puncture. AND serum separator tube. 
Specimen Preparation
CSF: Transport to local lab quickly and avoid pneumatic tube transport if possible. Protect from light during collection, storage and shipment. Centrifuge for 5 minutes ASAP or within 1 hour of collection. Transfer 2 mL CSF to an ARUP Amber Transport Tube. (Min: 1 mL) Do not freeze.
Serum: Protect from light during collection, storage and shipment. Allow specimen to clot completely at room temperature. Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Amber Transport Tube. (Min: 0.5 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Specimens not protected from light. Frozen CSF. 
CSF: Ambient: 4 hours; Refrigerated: 1 week; Frozen: Unacceptable
Serum: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months 
Reference Interval
Effective July 18, 2011
Test Number
Reference Interval
 Net Absorbance, Bilirubin, CSFEffective July 18, 2011
0.007 AU or less
 Net Absorbance, Oxyhemoglobin, CSFEffective July 18, 2011
0.020 AU or less
0020032Bilirubin, Total, Serum or PlasmaBy report

Interpretive Data
Refer to report.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
Component Test Code*Component Chart NameLOINC
2005249Net Absorbance, Bilirubin, CSF
2005250Net Absorbance, Oxyhemoglobin, CSF
2005252Serum Bilirubin1975-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Xanthochromia