Feedback
BCR-ABL1, Major (p210), Quantitative
2005017
Ordering Recommendation

This quantitative test is appropriate for diagnosis and therapeutic monitoring for CML or ALL. The BCR-ABL1 major (p210) fusion forms are present in almost all cases of CML and in a small subset of cases of ALL.

Mnemonic
BCR MAJ
Methodology
Quantitative Reverse Transcription Polymerase Chain Reaction
Performed
RNA isolation: Sun-Sat
Assay:
Mon, Wed-Fri
Reported
3-6 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or bone marrow (EDTA). Also acceptable: RNA extracted by CLIA certified lab. 
Specimen Preparation
Whole Blood: Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow:
Transport 3 mL bone marrow. (Min: 1 mL)
Specimens must be received within 48 hours of collection due to lability of RNA.
Extracted RNA:
Transport 40 uL RNA with at least 40 ng/uL concentration. (Min: 40 uL) Transport RNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. 
Storage/Transport Temperature
Whole Blood or Bone Marrow: CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.
Extracted RNA: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. 
Unacceptable Conditions
Serum, plasma, CSF, extracted DNA, RNA extracted by a non-CLIA lab, bone core, or FFPE tissue. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens. 
Remarks
 
Stability
Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable
Extracted RNA: Ambient: Unacceptable; Refrigerate: Unacceptable; Frozen: Indefinitely 
Reference Interval
Interpretive Data
Refer to report.

Compliance Statement D: For laboratory tests using a manufactured RUO kit. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
This test does not detect the rare BCR-ABL1 micro (p230) fusion form.

For the p190 fusion form (minor breakpoint), order BCR-ABL1, Minor (p190), Quantitative (ARUP test code 2005016).
Hotline History
View Hotline History
Components
Component Test Code*Component Chart NameLOINC
0051095BCR-ABL1, t(9;22) Source31208-2
2005012BCR-ABL1, Major (p210) Result42714-6
2005014BCR-ABL1, International Scale (Percent)69380-4
2005015EER BCR-ABL1, Major (p210)11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • CML RT-PCR
  • p210
  • Philadelphia Chromosome Ph1 Bone Marrow/Blood
  • t(9;22)