NRAS Mutation Detection by Pyrosequencing
2003123
Ordering Recommendation
Predicts response to anti-EGFR and MAPK pathway therapies in a variety of malignancies (eg, melanoma and colorectal cancer).
Mnemonic
NRAS
Methodology
Polymerase Chain Reaction/Pyrosequencing
Performed
Sun-Sat
Reported
10-12 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
- Patient Preparation
- Collect
- Tumor tissue.
- Specimen Preparation
- Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5 micron slides. (Min: 3 slides). Transport block and/or slide(s) in a tissue transport kit (ARUP supply # 47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800) 522-2787.
- Storage/Transport Temperature
- Room temperature. Ship in cooled container during summer months.
- Unacceptable Conditions
- Frozen specimens. Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Decalcified specimens.
- Remarks
- Include surgical pathology report. Tissue block will be returned after testing.
- Stability
- Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Reference Interval
Interpretive Data
Refer to report.
Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
Note
This assay detects mutations in codons 12, 13, and 61.
Hotline History
N/A
CPT Code(s)
88381; 81311
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 2002148 | Block ID | 57723-9 |
| 2003124 | NRAS Mutation Detection, Pyrosequencing | 21719-0 |
Aliases
- Anti-EGFR therapy resistance
- Codon 12,13
- Codon 61
- N-RAS
- RAS Mutation Analysis, Thyroid Cancer