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HLA-DR Genotyping
2002798
Ordering Recommendation

Do not use for specific disease screening or diagnosis (eg , celiac disease, rheumatologic diseases).

Mnemonic
HLA-DR DNA
Methodology
Polymerase Chain Reaction/Sequence Specific Oligonucleotide Probe Hybridization
Performed
Mon-Fri
Reported
3-7 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). 
Specimen Preparation
Transport 5 mL whole blood. (Min: 3 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Specimens collected in green (sodium or lithium heparin) tubes. 
Remarks
 
Stability
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data
Background Information for HLA-DR Genotyping:
Purpose: For immunization/vaccination trials or to aid the clinical diagnosis of diseases strongly associated with the HLA-DR locus.
Methodology: PCR followed by Sequence Specific Oligonucleotide Probe Hybridization of HLA-DR locus.
Analytical Sensitivity & Specificity: Medium to high resolution of HLA-DR locus.
Limitations: The presence of a disease-associated HLA combination does not establish a diagnosis. If less than 2 alleles are reported for a locus, the patient is likely homozygous. Rare diagnostic errors can occur due to primer or probe site mutations. This test is not sufficient for comprehensive HLA evaluation for clinical hematopoietic stem cell transplantation; for pre-transplant allele matching, consider HLA Class I (ABC) by Next Generation Sequencing (ARUP test code 2011264) and/or HLA Class II (DRB1 and DQB1) by Next Generation Sequencing (ARUP test code 2011272).
Occasionally the specific allele cannot be determined; in this case, the most likely allele assignment is made followed by a sequence of letters indicating other possible allele assignments. Interpretation of allele codes can be found at https://bioinformatics.bethematchclinical.org/hla/alpha.v3.html.

Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.

Note
Order this test for single antigen HLA-DR identification.  Please specify antigens.  Refer to HLA-DQB Genotyping for single antigen HLA-DQB identification.
Components
Component Test Code*Component Chart NameLOINC
2002780HLA Class II, Locus DRB1*, Allele 143290-6
2002781HLA Class II, Locus DRB1*, Allele 243290-6
2002847HLA-DR Genotyping Interpretation57298-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases

Performed at UUHC - H&I Lab