MLH1 Promoter Methylation, Paraffin
2002499
Ordering Recommendation
Distinguishes between Lynch syndrome and sporadic noncolorectal tumors with loss of MLH1.
Mnemonic
MLH1PCR
Methodology
Real-Time Polymerase Chain Reaction/Fluorescence Resonance Energy Transfer
Performed
DNA isolation: Sun-Sat
Assay:
Sun, Tue, Thu
Reported
10-12 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides) Transport block and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Frozen specimens. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Decalcified specimens.  
Remarks
Include surgical pathology report. Tissue block will be returned after testing.  
Stability
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
   
Interpretive Data
Refer to report.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

See Compliance Statement B: www.aruplab.com/CS
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
 
Note
 
CPT Code(s)
88381; 81479
Components
Component Test Code*Component Chart NameLOINC
0051749MLH1 Promoter Methylation 
2002148Block ID57723-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HNPCC screening
  • Lynch syndrome screening