EGFR Mutation Detection by Pyrosequencing
2002440
Ordering Recommendation
Predicts response to tyrosine kinase inhibitor (TKI) therapy.
Mnemonic
EGFR PCR
Methodology
Polymerase Chain Reaction/Pyrosequencing
Performed
DNA isolation: Sun-Sat
Assay:
Tue, Thu, Sat
Reported
10-12 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
For a general FNA collection and smear preparation refer to ARUP's Laboratory Test Directory: Cytology, Fine Needle Aspiration Collection at http://www.aruplab.com/guides/ug/tests/arup014.jsp  
Collect
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides) Tissue block will be returned after testing. A Fine Needle Aspirate (FNA) smear may also be submitted. Prepare FNA smear with Diff-Quik or equivalent stain by standard methods (air-dried slides are preferred). Number of slides needed is dependent on the tumor cellularity of the smear. (Min: 1 slide). Slide(s) will be destroyed during testing process and will not be returned to client.
Transport block and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Less than 25 percent tumor. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives. Decalcified specimens. FNA smears with less than 50 tumor cells.  
Remarks
  
Stability
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
   
Interpretive Data
Refer to report.

See Compliance Statement B: www.aruplab.com/CS  
Note
This test detects mutations in EGFR exons 18, 19, 20 and 21.
CPT Code(s)
88381; 81235
Components
Component Test Code*Component Chart Name
2002148Block ID
2002442EGFR by Pyrosequencing
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • EGFR-TKI
  • EGFR-tyrosine kinase inhibitor
  • Epidermal growth factor receptor
  • ERBB1
  • Erlotinib Responsiveness in NSCLC
  • Gefitinib Responsiveness in NSCLC
  • IRESSA Responsiveness in NSCLC
  • Non-small Cell Lung Cancer (NSCLC) Therapeutic Panel
  • Tarceva Responsiveness in NSCLC