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Osmotic Fragility, Erythrocyte
2002257
Ordering Recommendation

Functional testing of red blood cell sensitivity to osmotic stress; do not use to distinguish between spherocytes in hereditary spherocytosis and acquired autoimmune hemolytic anemia.

Mnemonic
OSM FRG
Methodology
Spectrophotometry
Performed
Mon-Fri
Reported
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Time SensitiveTime Sensitive
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Green (Sodium or Lithium Heparin) or Lavender (EDTA). 
Specimen Preparation
Transport 2 unfixed, air-dried, and unstained smears. (Min: 2 smears made from the blood submitted) AND 5 mL whole blood. (Min: 1 mL) Specimens should be refrigerated within 30 minutes after collection. Place both slides and whole blood specimens in an osmotic fragility transport kit (ARUP supply #53821) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Grossly hemolyzed specimens. 
Remarks
 
Stability
Ambient: Unacceptable; Refrigerated: 72 hours; Frozen: Unacceptable 
Reference Interval
Within normal curve limits. A graph will accompany laboratory report.
Interpretive Data
For patients with acute hemolysis, a normal red cell osmotic fragility test result cannot exclude an osmotic fragility abnormality since the osmotically labile cells may be hemolyzed and not present. Recommend testing during a state of prolonged homeostasis with stable hematocrit.

Components
Component Test Code*Component Chart NameLOINC
0049015Osmotic Fragility34964-7
2002142EER Osmotic Fragility11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • FRAGILITY, OSMOTIC (RBC)
  • Osmotic Fragility
  • RBC Fragility, Erythrocytes
  • Red Cell Fragility
  • Spherocytic Hemolytic Disease