Protein Electrophoresis with Reflex to Immunofixation Electrophoresis Monoclonal Protein Detection, Quantitation & Characterization, IgA, IgG, & IgM, Serum
2002109
Ordering Recommendation
Aids in the diagnosis and management of multiple myeloma and related disorders when clinical pretest probability is low. Use to detect and quantify monoclonal serum protein.
Submit With Order
Mnemonic
SPEP REFLEX
Methodology
Quantitative Capillary Electrophoresis/Qualitative Immunofixation Electrophoresis/Quantitative Nephelometry
Performed
Mon-Fri
Reported
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma.  
Remarks
 
Stability
After separation from cells: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 1 month  
Reference Interval
Test Number Components Reference Interval
0050640Protein Electrophoresis, Serum
Test Number Components Reference Interval
Total Protein-​Electrophoresis, Serum Refer to report
Albumin Refer to report
Alpha-​1 Globulins Refer to report
Alpha-​2 Globulins Refer to report
Beta Globulins Refer to report
Gamma Refer to report
0050350Immunoglobulin G
0-​ 30 days: 611-​1542 mg/dL
1 month: 241-​870 mg/dL
2 months: 198-​577 mg/dL
3 months: 169-​558 mg/dL
4 months: 188-​536 mg/dL
5 months: 165-​781 mg/dL
6 months: 206-​676 mg/dL
7-​8 months: 208-​868 mg/dL
9-​11 months: 282-​1026 mg/dL
1 year: 331-​1164 mg/dL
2 years: 407-​1009 mg/dL
3 years: 423-​1090 mg/dL
4 years: 444-​1187 mg/dL
5-​7 years: 608-​1229 mg/dL
8-​9 years: 584-​1509 mg/dL
10 years and older: 768-​1632 mg/dL
0050340Immunoglobulin A
0-​30 days: 1-​7 mg/dL
1 month: 1-​53 mg/dL
2 months: 3-​47 mg/dL
3 months: 5-​46 mg/dL
4 months: 4-​72 mg/dL
5 months: 8-​83 mg/dL
6 months: 8-​67 mg/dL
7-​8 months: 11-​89 mg/dL
9-​11 months: 16-​83 mg/dL
1 year: 14-​105 mg/dL
2 years: 14-​122 mg/dL
3 years: 22-​157 mg/dL
4 years: 25-​152 mg/dL
5-​7 years: 33-​200 mg/dL
8-​9 years: 45-​234 mg/dL
10 years and older: 68-​378 mg/dL
0050355Immunoglobulin M
0-​30 days: 0-​24 mg/dL
1 month: 19-​83 mg/dL
2 months: 16-​100 mg/dL
3 months: 23-​85 mg/dL
4 months: 26-​96 mg/dL
5 months: 31-​103 mg/dL
6 months: 33-​97 mg/dL
7-​8 months: 32-​120 mg/dL
9-​11 months: 39-​142 mg/dL
1 year: 41-​164 mg/dL
2 years: 46-​160 mg/dL
3 years: 45-​190 mg/dL
4 years: 41-​186 mg/dL
5-​7 years: 46-​197 mg/dL
8-​9 years: 49-​230 mg/dL
10 years and older: 60-​263 mg/dL
Interpretive Data
Immunofixation electrophoresis is a more sensitive technique than serum protein electrophoresis for the identification of small M-proteins found in patients with amyloidosis, early or treated myeloma and macroglobulinemia, MGUS, solitary plasmacytoma, and extramedullary plasmacytoma.
Note
If patterns from serum protein electrophoresis are monoclonal or suspicious, then Immunofixation Electrophoresis will be added. Additional charges apply.

IFE is a more sensitive method for determining and defining monoclonal proteins. No additional testing will be added for proteins that are normal, polyclonal, or that lack monoclonal patterns.
CPT Code(s)
84160; 84165; if reflexed, add 82784 x3 and 86334
Components
Component Test Code*Component Chart Name
0050020Albumin
0050035Alpha 1 Globulin
0050060Alpha 2 Globulin
0050110Beta Globulin
0050270Gamma
0050346Immunofixation Reflex
0050545Total Protein-Electrophoresis
2005168SPEP/IFE Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • Electrophoresis, Protein, Serum with Reflex to Immunofixation Electrophoresis, Serum
  • IFE Reflex
  • MGUS study
  • Monoclonal protein detection
  • Monoclonal Protein Study
  • Mutiple Myeloma Study
  • Protein Electrophoresis with Interpretation, Serum and Reflex to IFE and QNTIG's
  • Protein Electrophoresis, Serum with Reflex to IFE, Serum
  • Protein Electrophoresis, with Interpretation Serum, With Reflex to IFE, Serum
  • SPEP