Feedback
Chimerism, Recipient Pre-Transplant
2002065
Ordering Recommendation

Assess recipient genotype.

Mnemonic
STR-PRE
Methodology
Polymerase Chain Reaction/Fragment Analysis
Performed
Sun-Sat
Reported
5-30 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). OR bone marrow in lavender (EDTA). OR buccal brushes from recipient. 
Specimen Preparation
Transport 3 mL whole blood (Min: 1 mL) OR 1 mL bone marrow OR 2 buccal brushes in a sterile, dry tube. 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
 
Remarks
Post-transplantation results will be compared to pre-transplant recipient and donor genotypes, therefore, donor and recipient specimens must be obtained and genotyped before the transplant event occurs. 
Stability
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data
Background Information for Chimerism, Recipient Pre-Transplant:
Indication:
Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology:
PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Kit Used:
AmpFLSTR Identifiler® PCR Amplification Kit, Applied Biosystems.
Limit of Detection:
2 percent of minor cell population.
Limitations: Diagnostic errors can occur due to rare sequence variations.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Components
Component Test Code*Component Chart NameLOINC
0050758Chimerism, Recipient Pre, Interpretation
2001381Chimerism, Recipient Pre, Specimen
2002030Chimerism, Recipient Pre,InformativeLoci
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Bone marrow DNA typing
  • Chimerism evaluation
  • Pre transplant evaluation
  • Transplant analysis
  • Transplant evaluation