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Chimerism, Post-Transplant, Sorted Cells
2002064
Ordering Recommendation

Monitor engraftment of donor cells post allogenic stem cell transplantation. Each cell sort must have a unique ARUP accession number.

Mnemonic
STR-POSTSC
Methodology
Polymerase Chain Reaction/Fragment Analysis
Performed
Sun-Sat
Reported
5-9 days
New York DOH Approval Status
This test is New York DOH approved.
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). OR bone marrow in lavender (EDTA). 
Specimen Preparation
Transport 3 mL whole blood. (Min: 3 mL) OR 1 mL bone marrow. Ship overnight. If cell sorting is required, specimens should be received within 24 hours of collection for optimal isolation of the requested cell line(s). Increase the amount of blood/bone marrow submitted based on the number of cell lines requested and for patients with low cell counts. 
Storage/Transport Temperature
Ambient. 
Unacceptable Conditions
Clotted or hemolyzed specimens. 
Remarks
Post-transplantation genotypes will be compared to pre-transplant recipient and donor genotypes. Therefore, donor and recipient specimens must be obtained and genotyped before the transplant event occurs. Please provide the results and date of the patient's most recent WBC and differential counts. When submitting bone marrow specimens for cell sorting, please provide information regarding the general cellularity of the patient's bone marrow. See Cell Isolation Request for Chimerism, Post-Transplant, Sorted Cells. 
Stability
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable 
Reference Interval
Type Donor - Donor cells only.
Type Recipient - Recipient cells only.
Mixed - Donor and Recipient cells present.  Semi-quantitative results of percentage of donor and recipient cells will be reported.
Interpretive Data
Background Information for Chimerism, Post-Transplant, Sorted Cells:
Indication:
Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Kit Used:
AmpFLSTR Identifiler® PCR Amplification Kit, Applied Biosystems.
Limit of Detection:
2 percent of minor cell population.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
If requesting multiple cell lines, additional charges apply and vary based on the cell line requested (BMT Cell Isolation or BMT Chimerism Sort). Chimerism, Post-Transplant, Sorted Cells will be billed as separate tests for each cell line requested.
CPT Code(s)
81268; If sorted cells is performed, BMT will be billed additionally using CPT 88184; 88185
Components
Component Test Code*Component Chart NameLOINC
0051670Chimerism,Post-Cell Sort, Interpretation
2001380Chimerism,Post-Cell Sort, Specimen
2002034Chimerism,Post-Cell Sort,Margin of Error
2002035Chimerism,Post-Cell Sort, Percent Recip
2002036Chimerism,Post-Cell Sort, Percent Donor
2002037Chimerism,Post-Cell Sort,InformativeLoci
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Bone Marrow DNA typing
  • Bone Marrow DNA Typing Transplant Eval., Post-Transplant
  • Bone Marrow Post-Trans Eval
  • Bone Marrow Transplantation; Chimerism Evaluation
  • Engraftment Monitoring
  • Transplant Analysis
  • Transplant Evaluation