KRAS Mutation Detection with Reflex to BRAF Codon 600 Mutation Detection
Ordering Recommendation

Determine eligibility for anti-EGFR (cetuximab and panitumumab) therapy in patients with metastatic colorectal cancer.

Polymerase Chain Reaction/Pyrosequencing
DNA isolation: Sun-Sat
Assay: Mon, Wed, Fri
10-14 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Tumor tissue. 
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect paraffin block from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides). Transport block and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. 
Storage/Transport Temperature
Room temperature or refrigerated.  Ship in cooled container during summer months. 
Unacceptable Conditions
Frozen specimens. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Less than 25 percent tumor in tissue. Decalcified specimens. 
Include surgical pathology report. Tissue block will be returned after testing. 
bient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable 
Reference Interval
Interpretive Data
Refer to report.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

This test detects mutations in codons 12, 13, and 61. If KRAS is not detected, BRAF codon 600 Mutation Detection will be performed. Additional charges apply.
Hotline History
CPT Code(s)
88381; 81275; 81276; If reflexed, add 81210
Component Test Code*Component Chart NameLOINC
0040249KRAS Mutation Detection21702-6
2002148Block ID57723-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Anti-EGFR therapy eligibility
  • anti-EGFR therapy resistance testing
  • BRAF codon 600
  • Cetuximab (Erbitux)
  • KRAS codons 12, 13, 61
  • Panitumumab (Vectibix)
  • RAS