Laboratory Test Directory

Patient Preparation

Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.  

Collect

Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.  

Specimen Preparation

Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Infant Minimum: 3 mL (patient) AND 7 mL (control).  

Storage/Transport Temperature

CRITICAL ROOM TEMPERATURE.  

Unacceptable Conditions

Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.  

Remarks

 

Stability

Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable  

By report

Candida and tetanus antigens are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these antigens is determined by ³H-thymidine incorporation.

Results are reported as the counts per minute (CPM) antigen stimulated versus control cultures and a stimulation index (SI) which represents the ratio of mean CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.

See Compliance Statement B: www.aruplab.com/CS