Autoimmune Lymphoproliferative Profile
0095505
 
Ordering Recommendation
Mnemonic
ALPS
Methodology
Quantitative Flow Cytometry
Performed
Sun-Sat
Reported
1-3 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory.
Specimen Required
Patient Preparation
 
Collect
Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).  
Specimen Preparation
Transport 5 mL whole blood. (Min: 0.5 mL)  
Storage/Transport Temperature
CRITICAL ROOM TEMPERATURE.  
Unacceptable Conditions
Frozen or refrigerated specimens. Specimens older than 48 hours. Clotted or hemolyzed specimens.  
Remarks
Specimens must be analyzed within 48 hours of collection.  
Stability
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable  
Reference Interval
Effective November 18, 2013
Reports include age appropriate reference intervals and interpretation.
Available Separately Components Age: 0-​23 months Age: 2 years and older
No % Alpha/Beta+, CD4-​, CD8-​ 0-​5% 0-​7%
No Absolute Alpha/Beta+, CD4-​, CD8-​ 0-​340 cells/µL 0-​170 cells/µL
No % CD5+, CD20+ 0-​2% 0-​4%
No Absolute CD5+, CD20+ 0-​150 cells/µL 0-​100 cells/µL
No % CD3+, HLA-​DR+ 0-​8% 0-​11%
No Absolute CD3+, HLA-​DR+ 0-​600 cells/µL 0-​320 cells/µL
Interpretive Data
In Autoimmune Lymphoproliferative Syndrome (ALPS), all three of the lymphocyte subsets (alpha-beta positive, CD4 negative, and CD8 negative; CD5 positive and CD20 positive; and CD3 positive and HLA-DR positive) are elevated above the reference intervals. CD5 and HLA-DR may be found normally on B-cells and T-cells, respectively, as activation markers. Autoantibody production from CD5 positive B-cells is likely responsible for the autoimmune phenomena seen in ALPS. Increased levels of TCR alpha-beta positive, CD4 negative, and CD8 negative T-cells are the hallmark of ALPS and play a significant role in the pathophysiology of the disease.

See Compliance Statement A: www.aruplab.com/CS
Note
CPT Code(s)
86356 x7
Components
Component Test Code*Component Chart Name
0095516% Alpha/Beta+, CD4-, CD8-
0095517Absolute Alpha/Beta+, CD4-, CD8-
0095518% CD5+, CD20+
0095519Absolute CD5+, CD20+
0095521Absolute CD3+, HLA-DR+
0095985% CD3+, HLA-DR+
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Alpha-Beta+, Double Negative (CD4-, CD8-)
  • ALPS
  • Canale-Smith Syndrome
  • T-Cells for Autoimmune Lymphoproliferative Syndrome (ALPS)