Autoimmune Lymphoproliferative Profile
Ordering Recommendation
Quantitative Flow Cytometry
1-3 days  
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
Specimen Required
Patient Preparation
Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).  
Specimen Preparation
Transport 5 mL whole blood. (Min: 0.5 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Frozen or refrigerated specimens. Specimens older than 48 hours. Clotted or hemolyzed specimens.  
Specimens must be analyzed within 48 hours of collection.  
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable  
Reference Interval
Effective November 18, 2013
Reports include age appropriate reference intervals and interpretation.
Available Separately Components Age: 0-​23 months Age: 2 years and older
No % Alpha/Beta+, CD4-​, CD8-​ 0-​5% 0-​7%
No Absolute Alpha/Beta+, CD4-​, CD8-​ 0-​340 cells/µL 0-​170 cells/µL
No % CD5+, CD20+ 0-​2% 0-​4%
No Absolute CD5+, CD20+ 0-​150 cells/µL 0-​100 cells/µL
No % CD3+, HLA-​DR+ 0-​8% 0-​11%
No Absolute CD3+, HLA-​DR+ 0-​600 cells/µL 0-​320 cells/µL
Interpretive Data
In Autoimmune Lymphoproliferative Syndrome (ALPS), all three of the lymphocyte subsets (alpha-beta positive, CD4 negative, and CD8 negative; CD5 positive and CD20 positive; and CD3 positive and HLA-DR positive) are elevated above the reference intervals. CD5 and HLA-DR may be found normally on B-cells and T-cells, respectively, as activation markers. Autoantibody production from CD5 positive B-cells is likely responsible for the autoimmune phenomena seen in ALPS. Increased levels of TCR alpha-beta positive, CD4 negative, and CD8 negative T-cells are the hallmark of ALPS and play a significant role in the pathophysiology of the disease.

See Compliance Statement A:  
CPT Code(s)
86356 x7
Component Test Code*Component Chart Name
0095516% Alpha/Beta+, CD4-, CD8-
0095517Absolute Alpha/Beta+, CD4-, CD8-
0095518% CD5+, CD20+
0095519Absolute CD5+, CD20+
0095521Absolute CD3+, HLA-DR+
0095985% CD3+, HLA-DR+
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Alpha-Beta+, Double Negative (CD4-, CD8-)
  • Alpha/Beta positive
  • ALPS
  • Canale-Smith Syndrome
  • CD4 and CD8 negative
  • DNT
  • Double negative T cells
  • T-Cells for Autoimmune Lymphoproliferative Syndrome (ALPS)