DNA Content/Cell Cycle Analysis, Prostate
0095145
 
Ordering Recommendation
Mnemonic
DNA PROS
Methodology
Quantitative Flow Cytometry
Performed
Sun, Tue
Reported
3-9 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory.
Specimen Required
Patient Preparation
 
Collect
Prostate tumor tissue.  
Specimen Preparation
Paraffin embed tissue block enriched with prostate tumor. If no normal tissue is included in the block, supply a control tissue block to be processed in parallel.  
Storage/Transport Temperature
Refrigerated  
Unacceptable Conditions
No tumor tissue remaining on block. Specimens fixed in Bouin's solution (picric acid), mercuric chloride containing fixatives (e.g., B5, Zenker's solution), or ethanol-based fixatives containing ethylene glycol, acetic acid, or zinc chloride. Decalcified specimens.  
Remarks
Include H&E stained slide and surgical pathology report.  
Stability
Paraffin block: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Report components include: DNA content, S-phase interpretation, and copy of histogram.
Interpretive Data
In a study of 149 patients with prostate cancer, 63 percent were diploid and 37 percent were aneuploid. Five-year overall survival was 70 percent for diploid tumors and 42 percent for non-diploid tumors. Shorter survival correlated with reduced response to salvage hormone therapy for those previously exposed to short-term total androgen blockage. The significance of S-phase is controversial. [J Clin Oncol, 2003, 21:1238-1248; Prostate, 1997, 1:31 (1):21-8; Br J Cancer 1991, 64:578-82].

See Compliance Statement B: www.aruplab.com/CS
Note
A thin section of each tissue submitted is stained with H&E to verify the presence of tumor. The DNA content of each tumor is classified, in order of worsening prognosis, as diploid, tetraploid, aneuploid, or multiple aneuploid. The DNA index is the ratio of tumor G0--G1 cells to normal G0-G1 cells.

The tumor-specific S-phase is used when possible. An average histogram S-phase is used for diploid and aneuploid tumors where the tumor and host S-phases cannot be separated. An average histogram S-phase is also used when the percentage of aneuploid cells in the histogram is low (less than 25 percent). The S-phase interpretation is based on comparison to other prostate tumors and is controlled for histogram type (diploid, aneuploid). The appropriate population is divided into thirds and compared to the sample S-phase for an interpretation of low, moderate, or high S-phase.A thin section of each tissue submitted is stained with H&E to verify the presence of tumor. The DNA content of each tumor is classified, in order of worsening prognosis, as diploid, tetraploid, aneuploid, or multiple aneuploid. The DNA index is the ratio of tumor G0-G1 cells to normal G0-G1 cells.

The tumor-specific S-phase is used when possible. An average histogram S-phase is used for diploid and aneuploid tumors where the tumor and host S-phases cannot be separated. An average histogram S-phase is also used when the percentage of aneuploid cells in the histogram is low (less than 25 percent). The S-phase interpretation is based on comparison to other prostate tumors and is controlled for histogram type (diploid, aneuploid). The appropriate population is divided into thirds and compared to the sample S-phase for an interpretation of low, moderate, or high S-phase.
CPT Code(s)
88182
Components
Component Test Code*Component Chart Name
0095734DNA Content, Prostate
0095799DNA Content, Prostate, S-Phase Interp.
2008899DNA Index, Prostate
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • DNA Ploidy and S-Phase (Paraffin Block) (DNA Content/Cell Cycle Analysis, Prostate)