- Patient Preparation
- Colon tumor tissue.
- Specimen Preparation
- Paraffin embed tissue block enriched with colon tumor. If no normal tissue is included in the block, supply a control tissue block to be processed in parallel.
- Storage/Transport Temperature
- Unacceptable Conditions
- No tumor tissue remaining on block. Specimens fixed in Bouin's solution (picric acid), mercuric chloride containing fixatives (e.g., B5, Zenker's solution), or ethanol-based fixatives containing ethylene glycol, acetic acid or zinc chloride. Decalcified specimens.
- Include H&E stained slide and surgical pathology report.
- Paraffin block: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
The tumor-specific S-phase is used when possible. An average histogram S-phase is used for diploid and aneuploid tumors where the tumor and host S-phases cannot be separated. An average histogram S-phase is also used when the percentage of aneuploid cells in the histogram is low (less than 25 percent). The S-phase interpretation is based on comparison to other colon tumors and is controlled for histogram type (diploid, aneuploid). The appropriate population is divided into thirds and compared to the sample S-phase for an interpretation of low, moderate, or high S-phase.
|Component Test Code*||Component Chart Name||LOINC|
|0095733||DNA Content, Colon||30911-2|
|0095880||DNA Content, Colon, S-Phase Interp.|
|2008896||DNA Index, Colon|
- Breast Cancer aneuploidy
- DNA Ploidy and S-Phase (Paraffin Block)