CBFB-MYH11, inv(16) by RT-PCR
0092209
Ordering Recommendation
Use for detection of CBFB-MYH11 in acute myeloid leukemia (AML).
Mnemonic
INV(16)
Methodology
Reverse Transcription Polymerase Chain Reaction
Performed
Varies
Reported
4-7 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Lavender (EDTA) or bone marrow (EDTA).  
Specimen Preparation
Transport 5 mL whole blood or 3 mL bone marrow. (Min: 1 mL whole blood or 1 mL bone marrow) Specimens must be received within 48 hours of collection due to lability of RNA.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Serum or plasma. Frozen or clotted specimens. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed specimens.  
Remarks
  
Stability
Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable  
Reference Interval
   
Interpretive Data
Refer to report.

See Compliance Statement B: www.aruplab.com/CS
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
 
Note
CBFB (core binding factor β-chain)-MYH11 (myosin heavy chain 11 gene) fusion transcripts are detected in approximately 10% of de novo acute myelogenous leukemias (AML).  One half of these cases belong to AML subtype M4 with abnormal eosinophils (AML-M4Eo).  The fusion results from a pericentric inversion on chromosome 16 inv(16)(p13q22) or rarely from the translocation t(16;16)(p13;q22).  Based on the breakpoints in the CBFB and MYH11 genes, greater than 95% of analyzed inv(16) cases belong to three types: A (88%), D (5%), and E (5%).
CPT Code(s)
81401
Components
Component Test Code*Component Chart Name
0096364CBFB-MYH11, inv(16) by RT-PCR
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Aliases
  • CBFB-MYH11 inv(16) fusion gene
  • CBFB-MYH11 Inversion
  • inv(16)