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Amitriptyline and Nortriptyline, Serum or Plasma
0090158
Ordering Recommendation

Optimize drug therapy and monitor patient adherence.

Mnemonic
AMIT/NORT
Methodology
Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Performed
Mon, Wed, Fri
Reported
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Timing of specimen collection: Pre-dose (trough) draw - At steady state concentration. 
Collect
Plain red. Also acceptable: Lavender (K2 or K3EDTA) or pink (K2EDTA). 
Specimen Preparation
Separate serum or plasma from cells within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Whole blood. Gel separator tubes, light blue (citrate), or yellow (SPS or ACD solution). 
Remarks
 
Stability
After separation from cells: Ambient: 5 days; Refrigerated: 2 weeks; Frozen: 6 months 
Reference Interval
Effective February 19, 2013
Therapeutic RangeTotal (amitriptyline and nortriptyline): 95-250 ng/mL
Toxic LevelGreater than 500 ng/mL

Interpretive Data
Toxic concentrations may cause anticholinergic effects, cardiac abnormalities and seizures.

Note
Report includes individual values for amitriptyline, nortriptyline, and total.
CPT Code(s)
80335 (Alt code: G0480)
Components
Component Test Code*Component Chart NameLOINC
0090020Amitriptyline/Nortriptyline, Total, SP3335-7
0090024Amitriptyline, SP3333-2
0090210Nortriptyline, SP3872-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Amitriptyline/Nortriptyline Fractionation
  • Elatrol
  • Elavil
  • Endep
  • Etrafon
  • Laroxyl
  • Limbitrol
  • Saroten
  • Trepiline
  • Triavil
  • Tryptizol
  • Vanatrip