Testosterone, Females or Children
Ordering Recommendation
Use in conjunction with free testosterone in the evaluation of suspected hyperandrogenemia in women and children.
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Collect between 6-10 a.m.  
Serum separator tube or green (sodium or lithium heparin).  
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.2 mL)  
Storage/Transport Temperature
Unacceptable Conditions
EDTA plasma.  
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.  
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months  
Reference Interval
Effective August 19, 2013
Age Female Male
Premature (26-​28 weeks) 5-​16 ng/dL 59-​125 ng/dL
Premature (31-​35 weeks) 5-​22 ng/dL 37-​198 ng/dL
Newborn 20-​64 ng/dL 75-​400 ng/dL
1-​5 months Less than 20 ng/dL 14-​363 ng/dL
6-​24 months Less than 9 ng/dL Less than 37 ng/dL
2-​3 years Less than 20 ng/dL Less than 15 ng/dL
4-​5 years Less than 30 ng/dL Less than 19 ng/dL
6-​7 years Less than 7 ng/dL Less than 13 ng/dL
8-​9 years 1-​11ng/dL 2-​8 ng/dL
10-​11 years 3-​32 ng/dL 2-​165 ng/dL
12-​13 years 6-​50 ng/dL 3-​619 ng/dL
14-​15 years 6-​52 ng/dL 31-​733 ng/dL
16-​17 years 9-​58 ng/dL 158-​826 ng/dL
18-​39 years 9-​55 ng/dL 300-​1080 ng/dL
40-​59 years 9-​55 ng/dL 300-​890 ng/dL
60 years and older 5-​32 ng/dL 300-​720 ng/dL
Premenopausal (Greater than 18 years) 9-​55 ng/dL Does Not Apply
Postmenopausal 5-​32 ng/dL Does Not Apply
Tanner Stage I 2-​17 ng/dL 2-​15 ng/dL
Tanner Stage II 5-​40 ng/dL 3-​303 ng/dL
Tanner Stage III 10-​63 ng/dL 10-​851 ng/dL
Tanner Stage IV-​V 11-​62 ng/dL 162-​847 ng/dL
Interpretive Data
Total testosterone values may not reflect optimal concentrations in all individuals. Free or bioavailable testosterone measurements may provide supportive information.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0081058Testosterone, LC-MS/MS2986-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Total Testosterone