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Vitamin D, 1, 25-Dihydroxy
0080385
Ordering Recommendation

May be useful for evaluating calcium metabolism in individuals with hypercalcemia or renal failure in addition to Vitamin D, 25-Hydroxy testing. Test is not appropriate for diagnosing vitamin D deficiency or insufficiency.

Mnemonic
VIT D 1,25
Methodology
Quantitative Chemiluminescent Immunoassay
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube or plain red, lithium heparin or EDTA plasma. 
Specimen Preparation
Allow serum separator or plain red tube to sit for 15-20 minutes at room temperature for proper clot formation. Centrifuge and separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)
Storage/Transport Temperature: Refrigerated. 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Grossly hemolyzed or lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 6 months 
Reference Interval
19.9-79.3 pg/mL
Interpretive Data
This test is primarily indicated during patient evaluation for hypercalcemia and renal failure. A normal result does not rule out Vitamin D deficiency. The recommended test for diagnosing Vitamin D deficiency is Vitamin D 25-hydroxy.

Note
Hotline History
N/A
CPT Code(s)
82652
Components
Component Test Code*Component Chart NameLOINC
0080385Vitamin D, 1,25-Dihydroxy1649-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 1,25-(OH)2-D
  • 1,25-Dihydroxy Vitamin D
  • 1,25-Dihydroxycholecalciferol
  • 1,25-Dihydroxyvitamin D
  • 1,25-Dihydroxyvitamin D3
  • Calcitriol
  • D, 1-25 Dihydroxy, Vitamin
  • Vit D 1,25