Pyrazinamide (PZA) testing is suspended due to issues with the manufacturer’s kit. ARUP will continue testing the other first-line drugs: rifampin, isoniazid, and ethambutol. If PZA susceptibility testing is required during this test delay, contact your local public health laboratory or the CDC for pncA gene testing for PZA resistance mutations.

Ordering Recommendation

Identify acid-fast bacteria in blood or bone marrow specimens.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Aseptic draw.

Collect

Whole blood or bone marrow in Bactec® Myco/F Lytic bottle (ARUP supply # 31916) or yellow (SPS) tube (ARUP supply # 24964).

Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at 800-522-2787.

Specimen Preparation

Whole Blood: Transport 1-5 mL in Bactec® Myco/F Lytic bottle or transport 1-7mL in SPS yellow tube.
Bone Marrow:
Transport 1-5 mL (Min: 0.5 mL) in Bactec® Myco/F Lytic bottle or transport 1-7 mL (Min 0.5 mL) in SPS tube.

Storage/Transport Temperature

Room temperature

Unacceptable Conditions
Remarks
Stability

Ambient: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Methodology

Continuous Monitoring Blood Culture/Culture

Performed

Sun-Sat

Reported

1-43 days
Negative culture reported at 43 days. Positive culture reported as soon as detected.

Reference Interval

No growth

Interpretive Data



Compliance Category

FDA

Note

Identification and susceptibility testing is performed on positive cultures at an additional charge.

Hotline History

N/A

CPT Codes

87116; CPT codes vary based on method.

Components

Component Test Code* Component Chart Name LOINC
0060060 Culture, Blood for Acid Fast Bacilli 533-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • AFB blood culture
  • Culture, Blood for AFB
Blood Culture, Acid-Fast Bacillus (AFB)