Cyclosporine A, 2-Hour Post Dose (C2) by Tandem Mass Spectrometry
Ordering Recommendation

Optimize drug therapy and monitor patient adherence.

Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Two hour post-dose level should be drawn. 
Lavender (EDTA) or pink (K2EDTA). 
Specimen Preparation
Transport 1 mL whole blood. (Min: 0.25 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Serum or plasma. Specimens left at room temperature for longer than 24 hours. Clotted specimens. 
Ambient: 24 hours; Refrigerated: 2 weeks; Frozen: 2 months 
Reference Interval
Interpretive Data
Cyclosporine A levels in specimens drawn 2 hours post-dose may estimate the AUC better than trough specimens. The optimal therapeutic range for a given patient may differ from this suggested range based on the indication for therapy, treatment phase (initiation or maintenance), use in combination with other drugs, time of specimen collection relative to prior dose, type of transplanted organ, and/or the therapeutic approach of the transplant center. A suggested target range for renal transplant is 800-1700 ng/mL. A suggested target range for liver transplant is 600-1000 ng/mL. The higher ranges represent concentrations immediately post-transplant and the lower ranges represent concentrations during the maintenance phase.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Cyclosporine (Sandimmune) whole blood concentrations can be measured by either chromatographic or immunoassay methodologies. These two methodologies are not directly interchangeable, the measured cyclosporine whole blood concentration depends on the methodology used. Reference ranges may vary according to the specific immunoassay or HPLC-MS/MS test. Generally, immunoassays have been reported to have a positive bias relative to HPLC-MS/MS assays due to the detection of antibody cross-reactivity with cyclosporine metabolites.
Hotline History
Component Test Code*Component Chart NameLOINC
0058902CyclosporineA, 2-Hr Post(C2), HPLC-MS/MS32997-9
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