IGH-BCL2 (BCL-2/JH) Translocation, t(14;18) by PCR
0055616
 
Ordering Recommendation
Diagnosis of follicular lymphoma (FL) in conjunction with clinical, morphologic, and flow cytometric data.
Mnemonic
BCL2C-F
Methodology
Polymerase Chain Reaction
Performed
DNA isolation: Sun-Sat; Assay: Sun-Sat
Reported
2-7 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) OR bone marrow (EDTA) OR tissue.  
Specimen Preparation
Whole blood: Transport 5 mL. (Min: 1 mL) OR Bone marrow: Transport 3 mL. (Min: 1 mL) OR Fresh tissue: Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 tissue. OR FFPE tumor tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or four 10-micron shavings. Tissue block will be returned after testing. Transport block(s) and/or shavings in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Whole blood OR Bone marrow: Refrigerated. Fresh tissue: Frozen on dry ice. FFPE tumor tissue: Room temperature or refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Whole blood OR Bone marrow: Frozen specimens. Clotted or grossly hemolyzed specimens. FFPE tumor tissue: Specimens fixed/processed in alternative fixatives or heavy metal fixatives (B-4 or B-5) or tissue sections on slides. Decalcified specimens.  
Remarks
 
Stability
Whole blood OR Bone marrow: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Fresh Tissue:
Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year
FFPE tumor tissue:
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Interpretive Data
By report

See Compliance Statement B: www.aruplab.com/CS
Note
CPT Code(s)
81402
Components
Component Test Code*Component Chart Name
0055617IGH-BCL2 (BCL-2/JH) t(14;18) PCR, MBR
0055618IGH-BCL2 (BCL-2/JH) t(14;18) PCR, MCR
2006958IGH_BCL2, Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • BCL-2/JH translocation
  • IGH-BCL2 translocation